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Invitrogen™ TC-FlAsH™ TC-ReAsH™ II In-Cell Tetracysteine Tag Detection Kit, with Mammalian TC-Tag Gateway™ Expression Vectors (Green Fluorescence) (Red Fluorescence)
An expression tag-based fluorescence labeling system for live-cell labeling
Brand: Invitrogen™ T34563
Additional Details : Weight : 0.00450kg
Includes: The kit contains FlAsH-EDT2 labeling reagent, ReAsH-EDT2 labeling reagent, BAL wash buffer, pcDNA6.2/cTC-Tag-DEST, pcDNA6.2/nTC-Tag-DEST, and pcDNA6.2/nTC-Tag-p64 Control Plasmid.
Description
The expression construct is created when the Gateway™ entry clone (containing the gene of interest) is recombined with one of two destination vectors (pcDNA6.2/nTC-Tag-DEST for N-terminal tagging or pcDNA6.2/cTC-Tag-DEST for C-terminal tagging). The expression construct is then used to transform the host strain. The protein can be detected either with green-fluorescent FlAsH-EDT2 reagent or red-fluorescent ReAsH-EDT2 reagent. The tag is very small (6 amino acids) and the protein of interest is fluorescent only when the labeling reagent is added. BAL wash buffer replaces the previously supplied Disperse Blue 3 and EDT-based wash buffer as an olfactorily more agreeable reagent that yields superior signal to noise.
Cell Analysis, Cell-Based Reporter Assays, Cellular Imaging, Enzyme and Protein Activity Assays, Labeling Chemistry, Lumio Detection, Proteins, Expression, Isolation and Analysis, Reporter Assays, Tetracysteine (Lumio/FlAsH/ReAsH) Assays
Order Info
Shipping Condition: Wet Ice
Specifications
| Tetracysteine Tag Detection Kit | |
| Fluorescence Microscope | |
| Store in freezer (-5 to -30°C) and protect from light. | |
| FlAsH, ReAsH | |
| Kit |
| Fluorescent | |
| Tetracysteine Tag Detection | |
| Red, Green | |
| Wet Ice | |
| Gateway, TC-FlAsH II, TC-ReAsH II |
For Research Use Only. Not for use in diagnostic procedures.