
Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
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Gibco™ PBS, pH 7.4
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Gibco PBS, pH 7.4, is a balanced salt solution ideal for cell culture applications. It lacks calcium, magnesium, and phenol red, preventing cell adhesion and assay interference. Trusted for quality, Gibco PBS ensures reliable results in cell washing, transport, and reagent preparation.

Physical Form | Liquid |
---|---|
pH | 7.3 to 7.5 |
Chemical Name or Material | Phosphate Buffered Saline (PBS) |
Recommended Storage | 15°C to 30°C |
For Use With (Application) | Mammalian Cell Culture |
Gibco™ DPBS, no calcium, no magnesium
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DPBS, no calcium, no magnesium
Physical Form | Liquid |
---|---|
Chemical Name or Material | DPBS |
Recommended Storage | 15°to 30°C |
Gibco™ PBS, pH 7.4
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Gibco PBS, pH 7.4, is a balanced salt solution ideal for cell culture applications. It lacks calcium, magnesium, and phenol red, preventing cell adhesion and assay interference. Trusted for quality, Gibco PBS ensures reliable results in cell washing, transport, and reagent preparation.
Color | Clear |
---|---|
Physical Form | Liquid |
pH | 7.4 |
Chemical Name or Material | Phosphate Buffered Saline (PBS) |
For Use With (Application) | Washing Cells Before Dissociation; Diluting Cells for Counting and Preparing Reagents; Transporting Cells or Tissue |
Gibco™ DPBS, calcium, magnesium
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This product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
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DPBS, calcium, magnesium

Physical Form | Liquid |
---|---|
pH | 7 to 7.2 |
Chemical Name or Material | DPBS |
Recommended Storage | 2° to 8°C |
For Use With (Application) | Mammalian Cell Culture |
Gibco™ PBS (10X), pH 7.4
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Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.
Color | White |
---|---|
Physical Form | Liquid |
pH | 7.4 |
Chemical Name or Material | Phosphate Buffered Saline (PBS) |
Recommended Storage | Storage conditions: 15°C to 30°C Shipping conditions: Room temperature Shelf life: 24 months from date of manufacture |
For Use With (Application) | Mammalian Cell Culture |
Gibco™ PBS (10X), pH 7.4
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PBS (10X), pH 7.4
Physical Form | Liquid |
---|---|
pH | 7.3 to 7.5 |
Chemical Name or Material | Phosphate Buffered Saline (PBS) |
Recommended Storage | 15°to 30°C |
For Use With (Application) | Mammalian Cell Culture |
Gibco™ DPBS (10X), no calcium, no magnesium
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DPBS (10X), no calcium, no magnesium

Physical Form | Liquid |
---|---|
pH | 6 to 7 |
Chemical Name or Material | DPBS |
Recommended Storage | 15°to 30°C |
For Use With (Application) | Mammalian Cell Culture |
Physical Form | Liquid |
---|---|
Recommended Storage | Room temperature |
Gibco™ DPBS (10X), no calcium, no magnesium
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Dulbecco's phosphate-buffered saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.
Color | Clear |
---|---|
Physical Form | Liquid |
pH | 6.7 - 7.0 |
Chemical Name or Material | DPBS |
Recommended Storage | Storage conditions: 15°C to 30°C Shipping conditions: Room temperature Shelf life: 36 months from date of manufacture |
For Use With (Application) | Mammalian Cell Culture |
PBS Tablets, Phosphate Buffered Saline, Fisher BioReagents™
Phosphate Buffered Saline Tablets, CAS Number-7647-14-5, 7447-40-7, 100 tablets, Clear, Gray, White, 5/8 in., 600 SEC max., CAUTION!
Physical Form | Liquid |
---|---|
pH | 7.3 to 7.5 |
Packaging | Poly Cube |
Recommended Storage | Room temperature |
Gibco™ PBS, pH 7.2
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PBS, pH 7.2
Physical Form | Liquid |
---|---|
pH | 7.2 |
Chemical Name or Material | Phosphate Buffered Saline (PBS) |
Recommended Storage | 15°to 30°C |
For Use With (Application) | Mammalian Cell Culture |
Invitrogen™ PBS (Phosphate-Buffered Saline) Tablets
Phosphate-buffered saline (PBS) is a balanced salt formulation used for a variety of cellular applications.
Gibco™ DPBS, calcium, magnesium, glucose, pyruvate
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Dulbecco's Phosphate-Buffered Saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.
Physical Form | Liquid |
---|---|
Chemical Name or Material | DPBS |
For Use With (Application) | Mammalian Cell Culture |
Invitrogen™ PBS - Phosphate-Buffered Saline (10X) pH 7.4, RNase-free
PBS (phosphate buffered saline) is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions.
Physical Form | Liquid |
---|---|
pH | 7.4 |
Chemical Name or Material | Phosphate Buffered Saline (PBS) |
Recommended Storage | Store at room temperature. |