Protein Analysis Reagents
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Thermo Scientific™ Mass Spectrometry Grade Proteases
Improve sequence coverage and protein identification for mass spectrometry with high-quality, MS-grade proteases provided in stable liquid or lyophilized formats. Suitable for either in-solution or in-gel digestion workflows, each protease may be used individually or in combination to improve protein sequence coverage.
| Workflow Step | Protein Digestion |
|---|---|
| For Use With (Equipment) | Mass Spectrometer |
| Grade | MS |
| Detection Method | Mass Spectrometry |
| Product Line | Pierce |
Thermo Scientific™ Pierce™ BCA Protein Assay Kits
The Pierce BCA Protein Assay Kit is a high-precision, detergent-compatible protein assay for determination of protein concentration. Pierce BCA reagents provide accurate determination of protein concentration with most sample types encountered in protein research.
| Assay | BCA Assay |
|---|---|
| For Use With (Equipment) | Spectrophotometer, Microplate Reader |
| Product Type | Protein Quantitation Assay |
| For Use With (Application) | Solution-based Detection, Absorbance |
| Product Line | Pierce |
| Detection Method | Colorimetric |
Invitrogen™ Dynabeads™ Protein G for Immunoprecipitation
Dynabeads Protein G beads are uniform, 2.8-μm superparamagnetic beads with recombinant Protein G (∼17 kDa) covalently coupled to the surface.
| Shipping Condition | Ambient temperature |
|---|---|
| Content And Storage | Dynabeads Protein G are supplied in PBS, pH 7.4 w/0.01% Tween-20, 0.09% NaN3 Store at 2–8°C. |
| Purity or Quality Grade | For Research Use Only |
| Description | Recombinant Protein G covalently bound to Dynabeads |
| Surface Functionality | Protein G |
| Material | Polystyrene |
| Concentration | 30 mg/mL |
| For Use With (Application) | Immunoprecipitation |
| High-throughput Compatibility | High-throughput Compatible |
| Ligand Type | Protein G |
| Type | Superparamagnetic Beads |
| Target | Antibodies |
| Format | Beads in suspension |
| For Use With (Equipment) | KingFisher™ Sample Purification System, DynaMag™ magnets |
| Diameter (Metric) | 2.8 μm |
| Certifications/Compliance | ISO9001 and ISO13485 |
| Uniformity | Monosized 2.8 mm (CV <5%) |
| Product Line | Dynabeads |
Thermo Scientific™ Zeba™ Spin Desalting Columns, Plates, and Cartridges, 7K MWCO, 0.5–100 mL
Perform high-throughput protein recovery (7K MWCO) and sample desalting for mass spectrometry, HPLC, capillary electrophoresis, metabolite screening, and assay development with ready-to-use Thermo Scientific Pierce Zeba desalting chromatography columns, plates, and cartridges.
| MWCO | 7.0 kDa |
|---|---|
| Column Type | Size-exclusion, Proprietary Resin |
| Purification Target | Buffer Exchange, Protein |
| Product Line | Zeba |
Thermo Scientific™ Lysozyme
Degrade unwanted bacterial cell wall in protein or nucleic acid extracts
Cytiva™ Disposable PD-10 Desalting Columns
Use for desalting and buffer exchange, as well as removal of low-molecular weight compounds. Cytiva Life Sciences™ Disposable PD-10 Desalting Columns provide fast, high-recovery.
Thermo Scientific™ Halt™ Protease Inhibitor Cocktail (100X)
Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors.
| Buffer | Lysis Buffer |
|---|---|
| Content And Storage | Upon receipt store at 4°C |
| Inhibitors | Protease Inhibitor |
| Form | Liquid |
| Product Type | Protease Inhibitor Cocktail |
| Reagent Type | Protease Inhibitor |
| Product Line | Halt |
Thermo Scientific™ Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate), No-Weigh™ Format
Crosslink amines to sulfhydryls with this mid-length, water-soluble crosslinker made of NHS-ester and maleimide groups at ends of a cyclohexane-containing, 8-atom spacer arm.
| Shipping Condition | Ambient |
|---|---|
| Content And Storage | Upon receipt store desiccated at -20°C. |
| Description | Sulfo-SMCC |
| Cleavable | No |
| Water Soluble | Yes |
| Molecular Weight (g/mol) | 436.37 |
| Solubility | Water |
| Labeling Method | Chemical Labeling |
| Crosslinker Type | Heterobifunctional |
| Chemical Reactivity | Amine-Sulfhydryl |
| Cell Permeability | No |
| Format | Standard, Single-use, Premium-grade |
| Product Type | Crosslinker |
| Spacer | Short (<10 Å) |
| Reactive Moiety | Maleimide, Sulfo-NHS Ester |
| Spacer Arm Length | 8.3 Å |
| PEGylated | No |
Thermo Scientific™ Pierce™ Centrifuge Columns
Pack these empty 0.5 to 10 mL spin columns with agarose beads to perform small-scale affinity purification methods in 15- or 50-mL conical microcentrifuge tubes.
| Content And Storage | Store at room temperature. |
|---|---|
| Column Type | Empty Column, Polyethylene Filter |
| Product Line | Pierce |
Thermo Scientific™ Slide-A-Lyzer™ MINI Dialysis Devices, 10K MWCO
Perform sample buffer exchange with these 10,000-MW cut-off (MWCO) disposable polypropylene cups with integrated, low-binding membranes.
| Content And Storage | Store at room temperature |
|---|---|
| MWCO | 10 kDa |
| Format | MINI Dialysis Device |
| Membrane | Cellulose Membrane, Dialysis |
| Purification Target | Buffer Exchange, Protein |
| Product Line | Slide-A-Lyzer |
Invitrogen™ One Shot™ ccdB Survival™ 2 T1R Competent Cells
One Shot ccdB Survival 2 T1R Competent Cells are suitable for the propagation of plasmids containing the ccdB gene and are designed for use with the Gateway Vector Conversion System and for propagating Gateway destination, donor, and supercoiled entry vectors.
Invitrogen™ Alexa Fluor™ 488 NHS Ester (Succinimidyl Ester)
Alexa Fluor™ 488 is a bright, green-fluorescent dye with excitation ideally suited for the 488 nm laser line.
| Shipping Condition | Room Temperature |
|---|---|
| Content And Storage | Store in freezer (-5 to -30°C) and protect from light. |
| Product Type | Dye |
| Label or Dye | Alexa Fluor™ 488 |
| Emission | 517 nm |
| Excitation | 494 nm |
| Label Type | Alexa Fluor |
| Chemical Reactivity | Amine |
| Product Line | Alexa Fluor |
Invitrogen™ Dynabeads™ M-270 Epoxy
Dynabeads™ M-270 Epoxy beads are 2.8 μm superparamagnetic beads containing surface epoxy groups.
| Shipping Condition | Room Temperature |
|---|---|
| Type | Epoxy Bead |
| Purity or Quality Grade | Research Grade |
| Format | Freeze Dried |
| Surface Functionality | Epoxy |
| Sample Type | Any Sample Type |
| Diameter (Metric) | 2.8 μm |
| Shelf Life | 24 months from date of manufacture |
| Elution Volume Min | 5 μL |
| Product Line | DYNAL, Dynabeads |
Thermo Scientific™ Urea
Pure, crystalline urea (CH4N2O), CAS 57-13-6; denaturant with low UV-absorptivity for protein biology methods (elution, solubilization, refolding, storage).
Invitrogen™ Dynabeads™ Protein A for Immunoprecipitation
Dynabeads Protein A beads are uniform, 2.8-μm superparamagnetic beads with recombinant Protein A (∼45 kDa) covalently coupled to the surface.
| Shipping Condition | Ambient temperature |
|---|---|
| Content And Storage | Dynabeads Protein A are supplied in PBS, pH 7.4 w/0.01% Tween-20, 0.09% NaN3 Store at 2–8°C. |
| Purity or Quality Grade | For Research Use Only |
| Description | Recombinant Protein A covalently bound to Dynabeads |
| Surface Functionality | Protein A |
| Material | Polystyrene |
| Concentration | 30 mg/mL |
| For Use With (Application) | Immunoprecipitation |
| High-throughput Compatibility | High-throughput Compatible |
| Ligand Type | Protein A |
| Type | Superparamagnetic Beads |
| Target | Antibodies |
| Format | Beads in suspension |
| For Use With (Equipment) | KingFisher™ Sample Purification System, DynaMag™ magnets |
| Diameter (Metric) | 2.8 μm |
| Certifications/Compliance | ISO9001 and ISO13485 |
| Uniformity | Monosized 2.8 mm (CV <5%) |
| Product Line | Dynabeads |