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Invitrogen™ GSK3 beta (Total/Phospho) Multispecies InstantOne™ ELISA Kit

InstantOne ELISA

Brand:  Invitrogen™ 85-86173-11

Additional Details : Weight : 0.23750kg

Product Code. 15500817

This item is currently unavailable or has been discontinued.
View the product page for possible alternatives.

Alert:

The Total/Phospho InstantOne ELISAs contain reagents for a total of 96 wells (24 wells total/72 wells phospho). Please contact Technical Service for a lot specific COA.


Includes: User Manual
InstantOne™ Assay Plate - 96 well (sealed)
Cell Lysis Buffer (5X)
Enhancer Solution
Wash Buffer (10X)
GSK3b (Total) Capture Antibody Reagent
phospho-GSK3b (Ser9) Capture Antibody Reagent
GSK3b (Total) Detection Antibody Reagent
phospho-GSK3b (Ser9) Detection Antibody Reagent
Positive Control Cell Lysate (lyophilized)
Detection Reagent (TMB Substrate)
Stop Solution
Plate Seal

Description

Description

The InstantOne™ ELISA is specifically engineered for accurate measurement of total and phosphorylated human and mouse GSK3b (Ser9) in cell lysates. Detection of total and phosphorylated rat GSK3b (Ser9) is also expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.

Glycogen synthase kinase-3 beta (GSK3 beta) belongs to the subfamily of glycogen synthase kinases, that play a central role in energy metabolism, neuronal cell development, and body pattern formation. GSK3 has alpha and beta isoforms (51 kDa and 47 kDa respectively), that are functionally dissimilar. GSK3 beta plays a role in insulin and Wnt signaling, and is inhibited following the extracellular signaling events.
Specifications

Specifications

P49841,Q9WV60
ELISA Kit
Human, Mouse
Colorimetric Microplate Reader
Gsk3b
HRP
RUO
2°C to 8°C
Human, Mouse
1 hr. 10 min.
Biotin
Cell Lysates
ELISA
2932, 56637
Capture Antibody, Detection Antibody Reagent, InstantOne™ Assay Plate, Cell Lysis Buffer, Enhancer Solution, Wash Buffer (10X), Substrate Reagent (TMB), Stop Solution, Positive Control Cell Lysate (lyophilized), Plate seal
96 Tests
Cell Lysate,20 μL
Glycogen synthase kinase-3 beta
1 hr. 20 min.
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