Immunoassay Reagents and Kits
Immunoassay Reagents and Kits
Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.
We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.
ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.
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Invitrogen™ Cell Extraction Buffer
Suitable for use in ELISA and Western blotting
Invitrogen™ IFN gamma Human ELISA Kit
Each ready-to-use kit ELISA kit is validated for sensitivity, specificity, precision, and lot-to-lot consistency.
Target Species | Human |
---|---|
Conjugate | Biotin |
Interassay CV | 6.0% |
Intraassay CV | 5.5% |
Incubation Time | 2.75 hr. |
Gene Symbol | IFG,IFI |
Total Assay Time | 2 hr. 45 min. |
Sample Volume | Serum, 50 μL; Supernatant, 50 μL |
Assay Sensitivity | <4 pg/mL |
Storage Requirements | 2°C to 8°C |
Assay Range | 15.6-1000 pg/mL |
Marker Gene | IFN-γ |
For Use With (Application) | Colorimetric Detection |
Test Time | 1 hr. 20 min. |
Accession Number | P01579 |
Regulatory Status | RUO |
Target | Human |
Kit Contents | Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
For Use With (Equipment) | Colorimetric Microplate Reader |
Product Type | ELISA Kit |
Gene ID (Entrez) | 3458 |
Sample Type | Serum,Supernatant |
Label or Dye | HRP |
Invitrogen™ Cell Lysis Buffer II
Cell Lysis Buffer II is a high-quality, ready-to-use lysis buffer suitable for the preparation of cell extracts for ELISA, western blotting, and antibody bead immunoassays (Luminex) applications.
Target Species | Human |
---|---|
Conjugate | Biotin |
Gene Symbol | GMCSF |
Total Assay Time | 24 hr. 30 min. |
Sample Volume | Plasma, 100 μL; Serum, 100 μL; Supernatant, 100 μL |
Assay Sensitivity | 2 pg/mL |
Storage Requirements | 2°C to 8°C |
Assay Range | 2-250 pg/mL |
For Use With (Application) | ELISA |
Test Time | 1 hr. 45 min. |
Accession Number | P10145 |
Regulatory Status | RUO |
Target | Human |
Kit Contents | Capture Antibody: Pre-titrated; purified antibody,Detection Antibody: Pre-titrated; biotin-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,Coating Buffer: 10X PBS ELISA Coating Buffer,Assay Diluent: 5X Concentrated,Detection Enzyme: Pre-titrated Avidin-HRP,Substrate Solution: Tetramethylbenzidine (TMB) Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards.,96-well Plates: Corning Costar flat-bottom plates (included with Cat. Nos. ending in -22 and -86) |
For Use With (Equipment) | Colorimetric Microplate Reader |
Product Type | ELISA Kit |
Gene ID (Entrez) | 3576 |
Sample Type | Plasma,Serum,Supernatant |
Label or Dye | HRP |
Synonym | Interleukin-8, CXCL8, NAP-I |
Invitrogen™ IL-6 Human Uncoated ELISA Kit with Plates
Specifically engineered for accurate and precise measurement of human IL-6 protein levels from samples including serum, plasma, and cell culture supernatants.
Target Species | Human |
---|---|
Conjugate | Biotin |
Gene Symbol | CVID11,IL-21,Za11 |
Total Assay Time | 24 hr. 30 min. |
Sample Volume | Plasma, 100 μL; Serum, 100 μL; Supernatant, 100 μL |
Assay Sensitivity | 2 pg/mL |
Storage Requirements | 2°C to 8°C |
Assay Range | 2-200 pg/mL |
For Use With (Application) | ELISA |
Test Time | 1 hr. 45 min. |
Accession Number | P05231 |
Regulatory Status | RUO |
Target | Human |
Kit Contents | Capture Antibody: Pre-titrated; purified antibody,Detection Antibody: Pre-titrated; biotin-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Enzyme: Pre-titrated Streptavidin-HRP Concentrate (100X),Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22; -76; -86) |
For Use With (Equipment) | Colorimetric Microplate Reader |
Product Type | ELISA Kit |
Gene ID (Entrez) | 3569 |
Sample Type | Plasma,Serum,Supernatant |
Label or Dye | HRP |
Synonym | Interleukin-6 |
Invitrogen™ ProcartaPlex Human Basic Kit
Ideal solution for assessing multiple protein biomarkers in a single sample. This kit includes all non-target specific reagents - assay diluent, wash buffers, SA-PE and plates. Explore our broad portfolio of pre-configures panels and combinable single analytes.
Target Species | Human |
---|---|
Conjugate | Biotin |
Interassay CV | 8.1% |
Intraassay CV | 6.5% |
Gene Symbol | IGIF,IL-18,IL-1g,IL1F4 |
Total Assay Time | 3 hr. 10 min. |
Sample Volume | Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL |
Assay Sensitivity | 9.0 pg/mL |
Storage Requirements | 2°C to 8°C |
Assay Range | 78-5,000 pg/mL |
For Use With (Application) | ELISA |
Test Time | 1 hr. 20 min. |
Accession Number | Q14116 |
Regulatory Status | RUO |
Target | Human |
Kit Contents | Pre-coated 96 well plate,Adhesive Plate Covers,Sample Diluent,Assay Buffer concentrate,Assay Buffer concentrate,SAV-HRP,SAV-HRP,Wash Buffer,Chromogen,Chromogen,Stop Solution |
For Use With (Equipment) | Colorimetric Microplate Reader |
Product Type | ELISA Kit |
Gene ID (Entrez) | 3606 |
Sample Type | Plasma,Serum,Supernatant |
Label or Dye | HRP |
Synonym | Interleukin-18, Interferon-gamma-inducing factor |
Invitrogen™ Urea Nitrogen (BUN) Colorimetric Detection Kit
Assay Urea Nitrogen (BUN) levels in Serum, Plasma, Urine, Saliva and Tissue Culture Media.
Shipping Condition | Approved for shipment on Wet or Dry Ice |
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Immunoassay Kit Format | Colorimetric Detection Kit |
Content And Storage | 96-well plate(s), standard, other assay specific reagents, buffers, and lot-specific technical data sheet. Store at 2–8°C. |
For Use With (Equipment) | Microplate Reader |
Gene ID (Entrez) | Non-Gene |
Gene Symbol | Non-Gene |
Target Kit Named | Urea Nitrogen (BUN) |
Protein Family | Other Proteins |
---|---|
Target Species | Human |
Conjugate | Biotin |
Interassay CV | <12% |
Intraassay CV | <10% |
Gene Symbol | ATF,ATF1,ATF2,HFB1-GDNF,HSCR3 |
Total Assay Time | 4 hr. 45 min. |
Assay Sensitivity | 4 pg/mL |
Storage Requirements | 2°C to 8°C |
Research Discipline | Neurobiology |
For Use With (Application) | ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis |
Test Time | 1 hr. 20 min. |
Assay | Solid-phase sandwich ELISA |
Accession Number | P39905 |
Regulatory Status | RUO |
Target | Human |
Specificity | This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
For Use With (Equipment) | Colorimetric Microplate Reader |
Product Type | ELISA Kit |
Gene ID (Entrez) | 2668 |
Sample Type | Plasma,Serum,Supernatant |
Label or Dye | HRP |
Synonym | ATF1, ATF2, HFB1-GDNF, HSCR3 |
Invitrogen™ TNF alpha Human ELISA Kit
Each ready-to-use kit is validated for sensitivity, specificity, precision, and lot-to-lot consistency
Target Species | Human |
---|---|
Conjugate | Biotin |
Interassay CV | 7.5% |
Intraassay CV | 4.4% |
Incubation Time | 4 hr. |
Gene Symbol | DIF-alpha,TNFA,TNFSF2,TNLG1F,TNF |
Total Assay Time | 4 hr. |
Sample Volume | Plasma, 100 μL; Serum, 100 μL; Supernatant, 100 μL |
Assay Sensitivity | 1.7 pg/mL |
Storage Requirements | 2°C to 8°C |
Assay Range | 15.6-1000 pg/mL |
Marker Gene | TNF-α |
For Use With (Application) | Colorimetric Detection |
Test Time | 1 hr. 20 min. |
Accession Number | P01375 |
Regulatory Status | RUO |
Target | Human |
Kit Contents | Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
For Use With (Equipment) | Colorimetric Microplate Reader |
Product Type | ELISA Kit |
Gene ID (Entrez) | 7124 |
Sample Type | Plasma,Serum,Supernatant |
Label or Dye | HRP |
Invitrogen™ Interferon alpha (Cell culture) Human ELISA Kit
Measure levels of human interferon alpha (IFNa) in human serum and human plasma samples using 96-well plates.
Measurement | Absorbance at 450nm |
---|---|
Immunoassay Kit Format | Colorimetric ELISA (TMB substrate) with Plates |
Target Species | Human |
Conjugate | HRP |
Description | Human IFN alpha ELISA Kit (Serum Samples) |
Interassay CV | <8% |
Intraassay CV | <8% |
Gene Symbol | IFNA1,IFNA2 |
Total Assay Time | 3 hr. 15 min. |
Sample Volume | Supernatant, 100 μL |
Storage Requirements | 2°C to 8°C |
Assay Range | 12.5-1000pg/mL |
Test Time | 1 hr. 20 min. |
Regulatory Status | RUO |
Target | Human |
Specificity | No significant cross-reaction with human IFN-ß, IFN-g or IFN-w; mouse or rat IFN-a, IFN-ß, or IFN-g; bovine IFN-t |
Precision | Intra-assay CV ≤ 8%; inter-assay CV ≤ 8% |
For Use With (Equipment) | Colorimetric Microplate Reader |
Product Type | ELISA Kit |
Sample Type | Supernatant |
Label or Dye | HRP |
Target Species | Porcine |
---|---|
Conjugate | Biotin |
Interassay CV | 7.2% |
Intraassay CV | 5.1% |
Gene Symbol | CSIF,IL-10 |
Total Assay Time | 3 hr. |
Sample Volume | Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL |
Assay Sensitivity | <3 pg/mL |
Storage Requirements | 2°C to 8°C |
Assay Range | 7.8-500 pg/mL |
For Use With (Application) | Colorimetric |
Test Time | 1 hr. 20 min. |
Accession Number | Q29055 |
Regulatory Status | RUO |
Target | Porcine |
Kit Contents | Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
Precision | <3pg/mL |
For Use With (Equipment) | Colorimetric Microplate Reader |
Product Type | ELISA Kit |
Gene ID (Entrez) | 397106 |
Sample Type | Plasma,Serum,Supernatant |
Label or Dye | HRP |
Protein Family | Cytokines & Receptors |
---|---|
Conjugate | Biotin |
Interassay CV | <12% |
Intraassay CV | <10% |
Gene Symbol | IL6 |
Total Assay Time | 4 hr. 45 min. |
Sample Volume | Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL |
Assay Sensitivity | 45 pg/mL |
Storage Requirements | 2°C to 8°C |
Research Discipline | Immunology |
For Use With (Application) | ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis |
Test Time | 1 hr. 20 min. |
Detection Method | Colorimetric |
Assay | Solid-phase sandwich ELISA |
Accession Number | P26893 |
Regulatory Status | RUO |
Target | Porcine |
Specificity | The sandwich ELISA antibody pair detects Porcine IL-6. |
Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers,Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s) and stop solution |
For Use With (Equipment) | Colorimetric Microplate Reader |
Product Type | ELISA Kit |
Gene ID (Entrez) | 399500 |
Sample Type | Plasma,Serum,Supernatant |
Label or Dye | HRP |
Invitrogen™ Galactose Colorimetric Detection Kit
Assay Galactose levels in Serum, Plasma, Buffers and Tissue Culture Media.
Shipping Condition | Approved for shipment on Wet or Dry Ice |
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Immunoassay Kit Format | Colorimetric Detection Kit |
Content And Storage | 96-well plate(s), standard, other assay specific reagents, buffers, and lot-specific technical data sheet. Store at –20°C. |
For Use With (Equipment) | Microplate Reader |
Product Type | Galactose Colorimetric Detection Kit |
Gene ID (Entrez) | Non-Gene |
Sample Type | Plasma,Serum |
Gene Symbol | Non-Gene |
Target Kit Named | Galactose |
Detection Method | Colorimetric |