Biochemicals and Reagents

Applied Biosystems™ TaqMan™ Fast Universal PCR Master Mix (2X), No AmpErase™ UNG

TaqMan™ Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR. 5000 RXN FAST UNIVERSAL PCR MASTER MIX (2X), NOAmpErase UNG, 50mL 5000reactions Store at 4°C

Invitrogen™ RNAlater™ Stabilization Solution

Immediate RNase inactivation and RNA stabilization within tissues or cells RNALATER 20 X 5 ML W/MNL

Invitrogen™ RNaseOUT™ Recombinant Ribonuclease Inhibitor

Protects mRNA and improves total cDNA yields 5 KU RECOMBINANT.RIBONUCLEASE INHIBITOR RNASEOUT(Tm) Store at -20C

Invitrogen™ SYBR™ Safe™ DNA Gel Stain

Highly sensitive stain for visualization of DNA in agarose or acrylamide gels 400 UL DNA GEL STAIN, SYBR SAFE(TM), LIQUID 400µL

Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Gold Buffer and MgCl2

Chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation TAQ GOLD 5X5X1000U

Thermo Scientific™ GeneJET™ DNA Purification Kit

Perform silica membrane-based genomic DNA purification from mammalian cell culture and tissue samples, whole blood, bacteria and yeast. GENEJET GENOMIC KIT 250 PREP

Thermo Scientific™ GeneRuler™ 1kb Plus DNA Ladder

Perform sizing and approximate quantification of a wide range of double-stranded DNA on agarose gels. X25 GENERULER 1KB PLUS 250UG

Invitrogen™ E-Gel™ SizeSelect™ Agarose Gels Starter Kit, 2% (EU only)

E-Gel™ SizeSelect™ Agarose Gels Starter Kit, 2% (EU only) E-GEL SIZESELECT STARTER KIT

Thermo Scientific™ Phusion High-Fidelity DNA Polymerase

Get superior performance for cloning and other applications requiring high fidelity with one of the most accurate thermostable DNA polymerases available. 500 RXN PHUSION HIGH-FIDELITY PCR MASTER MIX GCbuffer formulation (10 x 1.25mL), 2X Master Mix

Invitrogen™ UltraPure™ 1 M Tris-HCI Buffer, pH 7.5

Pre-mixed, pH-adjusted, sterile-filtered solution 1 LT TRIS-HYDROCHLORIDE BUFFER SOLUTION PH 7.5 1Min plastic container, E-Z hold Store at 2-8C

Invitrogen™ MEGAscript™ SP6 Transcription Kit

Synthesize 10 to 50 times the amount of RNA produced by conventional transcription reactions 40 RXN MEGASCRIPT, SP6 KIT40REACTIONS STORE AT-20°C

Invitrogen™ RNaseZap™ RNase Decontamination Solution

Surface decontaminant that destroys RNases on contact 4 LT RNASE ZAP 4L STORE AT ROOM TEMPERATURE

Thermo Scientific™ GeneJET™ PCR Purification Kit

Perform rapid and efficient purification of DNA from PCR and other enzymatic reaction mixtures with this kit. GENEJET PCR PURIF 250PREP

Thermo Scientific™ Glycogen, RNA grade

Increase the recovery of nucleic acids during preciptation with this inert carrier. X2 reagents, RNA co-precipitant, Glycogen, from

Thermo Scientific™ GeneJET™ Plasmid Miniprep Kit

Perform isolation of high quality plasmid DNA from recombinant E. coli cultures with this simple, rapid, and cost-effective system. GENEJET PL MINIPREP 50PREP

Invitrogen™ TrackIt™ Cyan/Orange Loading Buffer

A 6X solution used for easy loading and tracking of DNA samples in agarose gels X3 Trackit(tm) cyan/orange buffer solution 500µL

Applied Biosystems™ Tempus™ Blood RNA Tube

Stabilize RNA and lyse whole blood cells in one step X50 Tempus(TM) blood RNA tube store at room temper

Thermo Scientific™ GeneRuler™ 100bp Plus DNA Ladder

Perform sizing and approximate quantification of a wide range of double-stranded DNA on agarose or polyacrylamide gels. X5 DNA ladders, GeneRuler(TM) 100 bp Plus DNA

Invitrogen™ SYBR™ Gold Nucleic Acid Gel Stain (10,000X Concentrate in DMSO)

Extremely sensitive fluorescent stain for detection of nucleic acids 500 UL NUCLEIC ACID GEL STAIN, SYBR(R) GOLD, MOSTsensitive stain for any gel system 500µL store at

Invitrogen™ Molecular Probes™ Quant-iT™ RNA HS Assay Kit

Provides an accurate and selective method for the quantitation of high-abundance RNA samples 1SET Qubit RNA HS Assay Kit 100AS, 5NG TO100ng, for use with Qubit fluorometer 00

Invitrogen™ dNTP Set (100 mM) Solution

Ready-to-use solution DNTP SET 100MM 4 X 250µMOL 00 STORE AT -20°C

Invitrogen™ RNase Away™ Decontamination Reagent

Ready-to-use solution for eliminating RNase and DNA contamination from labware 250 ML RNASE AWAY 250ML

Applied Biosystems™ RNase Inhibitor

No DNase or endonuclease activity 100 RXN RNASE INHIBITOR 100REACTIONS STORE AT -20C

Invitrogen™ 100 bp DNA Ladder

Suitable for sizing double-stranded DNA from 100 to 1,500bp DNA LADDER 100 BP STORE AT -20C

GE Healthcare illustra™ triplePrep Kit

Isolate gDNA, total RNA, and total denatured proteins from undivided animal tissues and mammalian cell samples in less than 1 hour 50 RXN ILLUSTRA TRIPLEPREP KIT 50 PREPS STORE ATroom temperature shelf life 150 days

Invitrogen™ SuperScript™ IV First-Strand Synthesis System

Optimized for synthesis of first-strand cDNA from purified poly(A)+ or total RNA. 200RXN SuperScript IV First-Strand Synthesis System

Water, Molecular Biology Grade, Fisher BioReagents

CAS: 7732-18-5 Molecular Formula: H2O Molecular Weight (g/mol): 18.015 InChI Key: XLYOFNOQVPJJNP-UHFFFAOYSA-N Synonym: dihydrogen oxide, dihydrogen monoxide PubChem CID: 962 ChEBI: CHEBI:15377 IUPAC Name: oxidane SMILES: O 10LT Water, Molecular Biology Grade (Sterile-Filtered)

Invitrogen™ MEGAscript™ T7 Transcription Kit

Ultra-high yield technology for in vitro transcription 25 RXN MEGASCRIPT, T7 KIT 25REACTIONS STORE AT-20°C

Invitrogen™ RNaseZap™ RNase Decontamination Wipes

Completely removes RNase contamination from glass and plastic surfaces RNASEZAP WIPESAmbion® RNaseZap Wipes are RNaseZap® in a

Invitrogen™ DNAZap™ PCR DNA Degradation Solutions

Instantanly degrades high levels of contaminating DNA and RNA from surfaces DNAZAP 2 X 250ML EA BOTTLEAmbion® DNAZap (patent pending) consists of two