DNA Reagents

Chemical reagents and other products used for DNA-related techniques and processes such as PCR cloning, DNA sequencing, DNA cloning and mapping, mutagenesis, DNA synthesis, etc.; includes kits, columns, buffers, labelling technology, and others.

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Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, without competent cells

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Specifications

Antibiotic Resistance Bacterial	Ampicillin (AmpR), Kanamycin (KanR)
Format	Kit
Product Type	Cloning Kit
Cloning Method	TOPO™-TA
Product Line	TOPO™

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Thermo Scientific™ Rapid DNA Ligation Kit

Enable fast sticky-end or blunt-end DNA ligation in only 5 minutes at room temperature.

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Specifications

Product Type	Rapid DNA Ligation Kit
Enzyme	DNA Ligase

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Thermo Scientific™ pUC19 DNA

Optimize cloning with this double-stranded closed circular high copy plasmid DNA, 2686 base pairs with a molecular weight of 1.74 x 10⁶ Da isolated from E.coli.

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Applied Biosystems™ 3500xL Genetic Analyzer 24-Capillary Array, 36 cm

Use for fragment analysis and rapid sequencing applications.

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Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, without competent cells

Pricing and Availability
Specifications

Promoter	SP6
Content And Storage	Box 1: • Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored.
Antibiotic Resistance Bacterial	Kanamycin (KanR), Zeocin™ (ZeoR)
Format	Kit
Product Type	PCR Cloning Kit
Cloning Method	Blunt TOPO™

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Applied Biosystems™ BigDye™ Terminator v3.1 Cycle Sequencing Kit

The BigDye™ Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.

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Specifications

Format	12-well Strips,8-well Strip,96- or 384-well Plate
Product Type	Sequencing Buffer
For Use With (Equipment)	SeqStudio™ Genetic Analyzer, SeqStudio™ Flex Series Genetic Analyzer, 3500/3500xL Genetic Analyzer, 3730/3730xl DNA Analyzer Thermal Cyclers: GeneAmp™ PCR System 9700 Dual Series, Veriti™ Fast 96‐well and 384‐well Thermal Cyclers
Technique	Fluorescent Dye Terminator Sequencing
Product Line	BigDye™ Terminator

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Invitrogen™ GeneArt™ Gibson Assembly HiFi Master Mix

GeneArt Gibson Assembly HiFi Master Mix enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15–60 minute isothermal reaction.

Pricing and Availability
Specifications

Shipping Condition	Ambient, Wet Ice, Dry Ice
Content And Storage	Includes master mix, positive control, and water and accommodates the use of your own competent cells.
Workflow Step	DNA Assembly
Number of Fragments	Up to 6 fragments
Product Type	HiFi Master Mix
Control Type	Positive Control
Cloning Method	Seamless cloning
For Use With (Application)	Seamless Cloning & Genetic Assembly
Product Line	GENEART™

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Thermo Scientific™ pUC18 DNA

Optimize cloning with this double-stranded closed circular high copy plasmid DNA, 2686 base pairs with a molecular weight of 1.74 x 10⁶ Da isolated from E.coli.

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Invitrogen™ GeneArt™ Gibson Assembly HiFi Cloning Kit, electrocompetent cells

The GeneArt Gibson Assembly HiFi Cloning Kit enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15–60 minute isothermal reaction.

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Applied Biosystems™ BigDye™ Direct Cycle Sequencing Kit

The BigDye™ Direct Cycle Sequencing Kit, part of the BigDye™ product family, is a complete kit for your PCR and Sequencing needs.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
Format	8-well Strip,96-well Plate
Product Type	Direct Cycle Sequencing Kit
Technique	Fluorescent Dye Terminator Sequencing
For Use With (Application)	Comparative Sequencing (Germline Mutations - 30:70 Heterozygotes), Comparative Sequencing (Germline Mutations - 50:50), GC-Rich Sequencing (>65%), GT-Rich or Difficult Template Sequencing, Standard Sequencing, Gap Closure, Homopolymer A or T, AT-Rich Sequencing (>65%), Comparative Sequencing (Germline Mutations -10:90 Heterozygotes)
Template Compatibility	BAC DNA, Cosmids, Lambda DNA, PCR Amplicons (10:90 Heterozygotes), Plasmid DNA (≤15Kb), Genomic DNA (Bacterial), Fosmids, PCR Amplicons (50:50 Heterozygotes), PCR Amplicons (30:70 Heterozygotes)
Product Line	BigDye™

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Thermo Scientific™ CloneJET PCR Cloning Kit

Efficiently clone PCR products generated with any thermostable DNA polymerase using Thermo Scientific™ CloneJET™ PCR Cloning Kit, a positive selection system.

Pricing and Availability
Specifications

Antibiotic Resistance Bacterial	Ampicillin (AmpR)
Format	Kit
Sample Type	PCR Products
Cloning Method	Restriction Enzyme⁄ MCS, Blunt PCR
For Use With (Application)	PCR Cloning
Vector	pJET1.2/blunt
Product Line	CloneJET

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Applied Biosystems™ Hi-Di™ Formamide

Highly deionized (Hi-Di) formamide

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Applied Biosystems™ 3730 Running Buffer (10X)

The concentrated Running Buffer is used for electrophoresis on Applied Biosystems™3730 and 3730XL DNA Analyzers.

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Thermo Scientific™ pENTR™/D-TOPO™ Cloning Kit

Utilize a highly efficient, 5-minute cloning strategy (TOPO™ Cloning) to directionally clone a blunt-end PCR product into a vector for entry into the Gateway™ System or the MultiSite Gateway™ System

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Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli cells

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of blunt-ended PCR products amplified with proofreading thermostable polymerases into a plasmid vector

Pricing and Availability
Specifications

Promoter	SP6
Content And Storage	Box 1: • Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid Store at -68 to -85°C.
Format	Kit
Product Type	PCR Cloning Kit
Cloning Method	Blunt TOPO™
Bacterial or Yeast Strain	TOP10
Cell Type	Chemically Competent

DNA Reagents

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Applied Biosystems™ 3730 Running Buffer (10X) Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

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Applied Biosystems™ 3730 Running Buffer (10X)