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  6. Invitrogen™ Collibri™ Library Quantification Kit

Invitrogen™ Collibri™ Library Quantification Kit

Product Code. 15930523 Shop All Thermo Scientific Products
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15930523 500 Reactions 500 reactions
15920523 100 Reactions Each
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Product Code. 15930523 Supplier Invitrogen™ Supplier No. A38524500

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Description

Invitrogen™ Collibri™ Library Quantification Kit includes a ready-to-use master mix optimized for Illumina NGS library quantification and a library dilution buffer

  • Includes Platinum II Taq Hot Start DNA Polymerase and enables convenient and accurate quantification of NGS libraries
  • Contains all components needed for the amplification reaction, including primers and passive reference dye, and does not require any preparation before use
  • Due to the universal concentration of the passive reference dye in the master mix, the kit can be used with any qPCR instrument without adjustments
  • Accurate quantification of libraries made from various sample types, including degraded samples
  • Ease of use with all components ready to use -- no reagent preparation required
  • Visual cues - specially formulated dyes included to track pipetting steps
  • Platinum hot-start technology - superior specificity, sensitivity, and room-temperature reaction setup Unmatched convenience
  • Contains:
    • Platinum II Taq Hot Start DNA Polymerase
    • Optimized buffer
    • dNTPs
    • Primer mix targeting adapter sequences
    • SYBR green dye
    • Passive reference dye
    • Blue dye to track pipetting steps
  • Unique formulation provides ease of use at every step of the workflow:
    • No reagent preparation
    • Tracking of pipetting steps
    • One cycling protocol for libraries of various lengths and GC/AT-content levels
    • Universal passive reference dye concentration - a single master mix for all qPCR instruments
  • Also includes a ready-to-use library dilution buffer that contains stabilizing agents to enable library integrity after dilution. The buffer contains yellow dye to track pipetting steps. When combined, the blue master mix and yellow diluted libraries become a green solution. This feature eases tracking of the addition of the diluted libraries or DNA standards to the master mix in the qPCR plate wells, helping reduce pipetting errors.
TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Store at -20°C.
Detection Method SYBR
Format Kit
Sample Type DNA, RNA
For Use With (Equipment) 7500 Fast System, 7900HT Fast System, QuantStudio™, ViiA™ 7 System, StepOne™ System, StepOnePlus™ System
GC-Rich PCR Performance Low
For Use With (Application) Illumina NGS Library Quantification
No. of Reactions 500 Reactions
PCR Method qPCR
Polymerase Platinum™ II Taq Hot Start DNA Polymerase
Product Line Collibri
Product Type Library Quantification Kit
Quantity 500 reactions
Shipping Condition Dry Ice
Test Time Standard
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Frequently Asked Questions (FAQs)

My qPCR instrument requires ROX. Which ROX dye do you recommend using with the Collibri Library Quantification Kit?

The Collibri Library Quantification Kit contains the Collibri Library Quantification Master Mix that already contains a universal concentration of ROX passive reference dye, so there is no need for additional adjustment with ROX dye regardless of the instrument you are working with.

Do I need to dilute the library prior to qPCR using the Collibri Library Quantification Kit?

Yes, the prepared library has to be diluted prior to running qPCR. Generally, it is recommended to run two dilution points, 1:10000 and 1:100000. For an established workflow where approximate library quantity is known, dilutions can be adjusted, but they need to fall within the range of the Collibri DNA Standards. Note that libraries should be diluted in the Collibri Library Dilution buffer that is included in the Collibri Library Quantification Kit. Dilution of the library stabilizes library molecules at low concentrations, producing accurate and reproducible results.
With the Collibri Library Quantification Kit, what effect do the visual cues for mixing have on library quantification results?

Colors in the Collibri Library Quantification Kit components have no effect on the universal passive reference dye, qPCR performance, or quantification results.

Can Collibri Library Quantification Kit be used with NGS libraries prepared for Ion Torrent sequencing?

No. However, the Collibri Library Quantification Kit contains the Collibri Library Quantification Master Mix that has premixed primers which are compatible with P5 and P7 adaptor sequences specific to the Illumina platform.

For Research Use Only. Not for use in diagnostic procedures.

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