Protein Purification
Cytiva HisTrap™ HP Prepacked Columns
Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
Thermo Scientific™ Halt™ Protease Inhibitor Cocktail (100X)
Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors.
Thermo Scientific™ Lysozyme Solution (50 mg/mL)
Increase protein or nucleic acid extraction efficiency with this enzyme that is characterized by the ability to break down the bacterial cell wall.
Thermo Scientific™ Pierce™ Chromogenic Endotoxin Quant Kit
Chromogenic Endotoxin Quant Kits are highly sensitive endpoint assays that measure and detect endotoxin (LPS) in protein, peptide, nucleic acid, or antibody samples using the Amebocyte Lysate Assay from 0.01–1.0 EU/mL. Kits are available in reaction sizes ranging from 30 to 240.
IBA Lifesciences Strep-Tactin™XT 4Flow™ Resin
Strep-Tactin™XT 4Flow™ resin is a 50% suspension to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins
Cytiva PD MiniTrap™ G-25 Sample Preparation Columns
Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 0.5mL. Cytiva Life Sciences™ D MiniTrap™ G-25 Sample Preparation Columns are convenient and prepacked.
Gibco™ AAV-MAX Lysis Buffer
AAV-MAX Lysis Buffer is a ready-to-use cell lysis reagent for the extraction of adeno-associated virus (AAV) particles from producer HEK293 cells. Cell lysis is detergent-based, eliminating the need for mechanical cell disruption.
Thermo Scientific™ Halt™ Protease and Phosphatase Inhibitor Cocktail (100X)
Prevent protein degradation and stabilize phosphorylation with this all-in-one cocktail of protease and phosphatase inhibitors.
Proteintech ChromoTek GFP-Trap™ Magnetic Agarose
The ChromoTek GFP-Trap™ Magnetic Agarose are affinity beads for IP of GFP-fusion proteins.
Thermo Scientific™ Brij™-35, 30% Solution
Stabilized Brij-35 detergent provided as 10% and 30% solutions for use in various protein methods including cell lysis and HPLC.
Thermo Scientific™ Pierce™ High Capacity Streptavidin Agarose
Bind biotinylated proteins and other ligands with a wide selection of high-quality agarose and acrylamide beads and columns of recombinant streptavidin biotin-binding protein.
Thermo Scientific™ High-Purity Maltoside Detergents
Maltoside detergents are a class of nonionic surfactants that are commonly utilized in the field of membrane protein solubilization. Several studies have shown that these surfactants are highly effective in extracting and stabilizing membrane proteins.
Invitrogen™ Dynabeads™ Protein G Immunoprecipitation Kit
Dynabeads Protein G Immunoprecipitation Kit is intended for immunoprecipitation (IP) and includes all reagents and buffers required to perform IP using your own antibody.
Thermo Scientific™ Detergent Removal Spin Columns & Plates
Rapidly eliminate high concentrations of detergents with minimal sample loss with detergent removal resins. Thermo Scientific HiPPR (High Protein and Peptide Recovery) resin is recommended for protein concentrations up to 100 μg/mL, while Thermo Scientific Pierce resin is suitable for concentrations over 100 μg/mL.
Thermo Scientific™ HisPur™ Ni-NTA Spin Columns, 0.2 mL
Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.