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Protein Purification

Kits, columns, gels, reagents, enzymes, inhibitors, and other assorted products for use with protein purification procedures.
Protein Purification Reagents and Kits (1,641)
Magnetic Affinity Separation Particles (259)
Protease Inhibitors and Phosphatase Inhibitors (131)
Spin Columns and Gravity-flow Columns (119)
Gel Filtration Media and Columns (116)
Miscellaneous Immobilization Supports (110)
Cell Lysis Detergents (78)
In vitro Protein Expression Systems (41)
Affinity Gels (36)
Biotin-Binding Affinity Resins and Beads (34)
Miscellaneous Immobilized Affinity Ligands (29)
Cell Lysis Enzymes (17)

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1 – 15 of 1,625 results
1
Promotions Available

Thermo Scientific™ Halt™ Protease Inhibitor Cocktail (100X)

Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors.

EDGE
Buffer Lysis Buffer
Content And Storage Upon receipt store at 4°C
Inhibitors Protease Inhibitor
Form Liquid
Product Type Protease Inhibitor Cocktail
Reagent Type Protease Inhibitor
Product Line Halt™
2

Cytiva HisTrap™ HP Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

EDGE
Flow Rate 4 mL/min. (1 mL), 5 mL/min. (5 mL)
Type Pre-packed Column
Content And Storage 4°C to 30°C
Description HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery.
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Material Polypropylene
Support Type 6% Highly Cross-linked Agarose
3
Promotions Available

Thermo Scientific™ Lysozyme Solution (50 mg/mL)

Increase protein or nucleic acid extraction efficiency with this enzyme that is characterized by the ability to break down the bacterial cell wall.

EDGE
4

IBA Lifesciences Strep-Tactin™XT 4Flow™ Resin
BIOPHARMA

Strep-Tactin™XT 4Flow™ resin is a 50% suspension to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins

EDGE
5
Promotions Available

Thermo Scientific™ Pierce™ Chromogenic Endotoxin Quant Kit

Chromogenic Endotoxin Quant Kits are highly sensitive endpoint assays that measure and detect endotoxin (LPS) in protein, peptide, nucleic acid, or antibody samples using the Amebocyte Lysate Assay from 0.01–1.0 EU/mL. Kits are available in reaction sizes ranging from 30 to 240.

Shipping Condition Wet Ice
Assay End-point Chromogenic Assay
Type Endotoxin Quantitation Kit
Target Endotoxins
Content And Storage • Lyophilized amebocyte lysate
• E.coli endotoxin standard
• Chromogenic substrate
• Endotoxin-free water

Store at 2–8°C upon receipt.
Format Kit
Assay Sensitivity 0.01 EU/mL
Assay Range 0.01 to 0.1 EU/mL, 0.1 to 1.0 EU/mL
Product Line Pierce™
Detection Method Colorimetric
6

Cytiva PD MiniTrap™ G-25 Sample Preparation Columns

Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 0.5mL. Cytiva Life Sciences™ D MiniTrap™ G-25 Sample Preparation Columns are convenient and prepacked.

7

Invitrogen™ Dynabeads™ Protein A for Immunoprecipitation

Dynabeads Protein A beads are uniform, 2.8-μm superparamagnetic beads with recombinant Protein A (∼45 kDa) covalently coupled to the surface.

EDGE
Shipping Condition Ambient temperature
Content And Storage Dynabeads Protein A are supplied in PBS, pH 7.4 w/0.01% Tween-20, 0.09% NaN3 Store at 2–8°C.
Purity or Quality Grade For Research Use Only
Description Recombinant Protein A covalently bound to Dynabeads
Surface Functionality Protein A
Material Polystyrene
Concentration 30 mg/mL
For Use With (Application) Immunoprecipitation
High-throughput Compatibility High-throughput Compatible
Ligand Type Protein A
Type Superparamagnetic Beads
Target Antibodies
Format Beads in suspension
For Use With (Equipment) KingFisher™ Sample Purification System, DynaMag™ magnets
Diameter (Metric) 2.8 μm
Certifications/Compliance ISO9001 and ISO13485
Uniformity Monosized 2.8 mm (CV <5%)
Product Line Dynabeads™
8
Promotions Available

Thermo Scientific™ Pierce™ Spin Columns - Snap Cap

Perform small-scale affinity methods, such as immunoprecipitation assays with agarose beads using these empty spin columns and cups (5 to 500μL).

EDGE
9

Proteintech ChromoTek GFP-Trap™ Magnetic Agarose

The ChromoTek GFP-Trap™ Magnetic Agarose are affinity beads for IP of GFP-fusion proteins.

Target AcGFP, Clover, eGFP, Emerald, GFP, GFP5, GFP Envy, GFP S65T, mGFP, mPhluorin, PA-GFP, Superfolder GFP, TagGFP, TagGFP2, monomeric eGFP A206K, CFP, YFP, Citrine, eCitrine, eYFP, Venus, Ypet, BFP
Content And Storage Upon receipt store at 4°C. Do not freeze.
Binding Capacity 10 μL slurry bind more than 8 μg of recombinant GFP
Label or Dye Magnetic Agarose
For Use With (Application) IP, CoIP, ChIP, RIP
Species Alpaca
10
Promotions Available

Thermo Scientific™ Pierce™ Protein G Magnetic Beads

Perform antibody purification and immunoprecipitation methods using manual or robotic magnetic separators with these high-capacity and high-throughput affinity particles.

EDGE
Particle Size 1 μm
Ligand Type Protein G
Type Magnetic Affinity Bead
Target Antibody
Content And Storage Upon receipt store at 4°C. Product shipped with an ice pack.
Protein Form Recombinant
Form Liquid
Concentration Slurry: 10mg/mL, 1% solids
Product Line Pierce™
11
Promotions Available

Thermo Scientific™ Pierce™ Anti-DYKDDDDK Magnetic Agarose

Thermo Scientific™ Pierce™ Anti-DYKDDDK Magnetic Agarose Beads provide a fast, convenient method for purification of DYKDDDK-tagged recombinant proteins

EDGE
dimMatrix Magnetic Agarose
12
Promotions Available

Invitrogen™ Dynabeads™ Protein G Immunoprecipitation Kit

Dynabeads Protein G Immunoprecipitation Kit is intended for immunoprecipitation (IP) and includes all reagents and buffers required to perform IP using your own antibody.

EDGE
13
Promotions Available

Thermo Scientific™ HisPur™ Ni-NTA Spin Columns, 0.2 mL

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

EDGE
14
Promotions Available

Thermo Scientific™ Pierce™ High Capacity Streptavidin Agarose

Bind biotinylated proteins and other ligands with a wide selection of high-quality agarose and acrylamide beads and columns of recombinant streptavidin biotin-binding protein.

EDGE
Particle Size 45 to 165 μm
Stationary Phase Streptavidin
Type Agarose
Target Biotin-Tagged Molecule
Content And Storage Upon receipt store at 4°C. Do not freeze the resin. Product is shipped at ambient temperature.
Format Bottle
Protein Form Recombinant
Column Type Affinity, Agarose Resin
Sufficient For Binding ≥/= 100 μg Biotin/mL Resin (≥/= 10 mg Biotinylated BSA/mL Resin)
Product Line Pierce™
15
Promotions Available

Invitrogen™ DynaGreen™ Protein A/G Magnetic Beads
GREENER_CHOICE

DynaGreen Protein A/G Magnetic Beads are intended for use in immunoprecipitation (IP) applications while also being an environmentally sustainable choice.

EDGE
Shipping Condition Ambient temperature
Content And Storage DynaGreen Protein A/G Magnetic Beads are supplied in phosphate buffer, pH 7.4, with a biodegradable surfactant and a preservative. Store at 2–8°C.
Purity or Quality Grade For Research Use Only
Description Recombinant Protein A/G covalently bound to DynaGreen magnetic beads
Color Brown
Material Iron Oxide
Green Features Less hazardous, less waste, sustainable packaging
Concentration 20 mg/mL
For Use With (Application) Immunoprecipitation
High-throughput Compatibility High-throughput Compatible
Ligand Type Recombinant Protein A/G
Type Protein A/G Magnetic Bead
Target Proteins
For Use With (Equipment) KingFisher™ Sample Purification Systems, DynaMag™ Magnets
Diameter (Metric) 250 nm
Certifications/Compliance ISO9001 and ISO13485
Shelf Life 12 months from date of manufacture
Product Line DynaGreen™