Real Time PCR Master Mixes

Real-time PCR master mixes are batch mixtures of PCR reagents at optimal concentrations that can be prepared and divided among multiple PCR tubes/plates. They reduce pipetting and risk of contamination, save time, and limit possible mixing errors.

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Applied Biosystems™ SYBR™ Green Universal Master Mix

Everything needed for SYBR™ Green dye-based PCR amplification and detection in a convenient, single-tube format.

Pricing and Availability
Specifications

Polymerase	AmpliTaq Gold DNA Polymerase
GC-Rich PCR Performance	High
Format	Tube
Product Type	Real Time PCR SYBR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
Reaction Speed	Standard
Label or Dye	SYBR Green
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Product Line	SYBR™
Detection Method	SYBR

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Applied Biosystems™ TaqMan™ Genotyping Master Mix

Optimized for end-point fluorescence detection in SNP genotyping applications.

Pricing and Availability
Specifications

GC-Rich PCR Performance	High
Fidelity (vs. Taq)	2 X
Purity or Quality Grade	UP (Ultra Pure)
Concentration	2X
For Use With (Application)	Genotyping
PCR Method	qPCR
Detection Method	Primer-probe
Polymerase	AmpliTaq Gold DNA Polymerase
Genotyping Target	Copy Number Variants, SNPs (Unknown or Numerous), Drug Metabolizing Enzymes, SNPs (Known)
Product Type	Genotyping Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7
Sample Type	DNA (Genomic)
Reaction Speed	Standard
Technique	SNP Genotyping (Real-Time PCR-Based), CNV (Copy Number Variation)
Product Line	TaqMan™

Applied Biosystems™ TaqPath™ 1-Step RT-qPCR Master Mix, CG

TaqPath 1-Step RT-qPCR Master Mix, CG, is designed for robust and reproducible one-step pathogen detection and gene expression workflows. The single-tube 4X formulation contains thermostable MMLV reverse transcriptase, dNTPs, UNG, ROX dye, and thermostable Fast DNA polymerase.

Pricing and Availability
Specifications

Reverse Transcriptase	M-MLV
Form	Liquid
InhibitorToleranceSimple	High
Concentration	4X
For Use With (Application)	Microbial Detection,DNA Quantitation,Presence/Absence
PCR Method	1-step RT-qPCR
Multiplex Capability	Up to 3-plex
Detection Method	Primer-probe
Includes	dUTP, UNG/UDG
Thermostability	72 hr., Assembled Reaction
Polymerase	Fast DNA Polymerase
Sensitivity	Down to 10 copies
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System, 7500 Fast Dx System, QuantStudio™ Dx
Sample Type	RNA, cDNA, DNA
Reaction Speed	Fast or Standard
Target Specificity	RNA only, Both RNA and DNA
Hot Start	Built-In Hot Start
Product Line	TaqPath™

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Applied Biosystems™ TaqPath™ qPCR Master Mix, CG

TaqPath qPCR Master Mix, CG, is a 2X formulation designed for gene expression and miRNA analysis. It contains thermostable Fast DNA polymerase, uracil-N-glycosylase (UNG), dNTPs with dUTP, ROX dye (passive reference), and optimized buffer components for maximum robustness and reproducibility.

Pricing and Availability
Specifications

Content And Storage	Store at 2°C to 8°C in the dark.
Form	Liquid
InhibitorToleranceSimple	High
Concentration	2X
For Use With (Application)	Gene Expression,Microbial Detection,DNA Quantitation,Presence/Absence,miRNA analysis
PCR Method	qPCR
Multiplex Capability	Duplex
Detection Method	Primer-probe
Includes	dUTP, UNG/UDG
Thermostability	48 hr., Assembled Reaction
Polymerase	Fast DNA Polymerase
Sensitivity	Down to 10 copies
Format	Bottle
Product Type	qPCR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	QuantStudio™ Dx, 7500 Fast Dx, QuantStudio™ Absolute Q Digital PCR System
Sample Type	cDNA, DNA
Reaction Speed	Fast or Standard
Target Specificity	cDNA or gDNA only
Hot Start	Built-In Hot Start
Product Line	TaqPath™

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Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR

Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.

Pricing and Availability
Specifications

Shipping Condition	Wet Ice
InhibitorToleranceSimple	Medium
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Multiplex Capability	Single-plex
Detection Method	SYBR
Includes	dUTP, UNG/UDG
Thermostability	72 hr., Assembled Reaction
Polymerase	Dual-Lock Taq DNA Polymerase
Format	Tube
Product Type	Real Time PCR SYBR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 7 Flex, QuantStudio™ Absolute Q Digital PCR System, StepOne™, StepOnePlus™, ViiA™ 7 System
Sample Type	DNA
Reaction Speed	Fast or Standard
Target Specificity	cDNA or gDNA only
Product Line	PowerUP™, SYBR™

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Applied Biosystems™ Path-ID™ qPCR Master Mix

Ambion™ Path-ID™ qPCR Master Mix is a convenient enzyme, buffer, and dNTP mix for TaqMan™ probe-based, real-time PCR detection of DNA targets.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
Content And Storage	2X qPCR Master Mix is stored at –20°C. Nuclease-free water may be stored at any temperature.
Purity or Quality Grade	UP (Ultra Pure)
Form	Liquid
For Use With (Application)	Animal Health,Pathogen Detection
PCR Method	qPCR
Detection Method	Primer-probe
Polymerase	AmpliTaq Gold DNA Polymerase
Format	Tube
Passive Reference Dye	ROX (Pre-mixed)
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Fast or Standard
Hot Start	Built-In Hot Start
Product Line	Ambion™, Path-ID™

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Applied Biosystems™ TaqMan™ Universal Master Mix II, no UNG

TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR).

Pricing and Availability
Specifications

GC-Rich PCR Performance	High
Content And Storage	Store at 2°C to 8°C in the dark. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Fidelity (vs. Taq)	2 X
Purity or Quality Grade	UP (Ultra Pure)
Concentration	2X
For Use With (Application)	Gene Expression,Genotyping,miRNA analysis
PCR Method	qPCR
Detection Method	Primer-probe
Polymerase	AmpliTaq Gold DNA Polymerase
Product Type	Universal Master Mix II
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7300 System, 7500 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Standard
Product Line	TaqMan™

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Applied Biosystems™ TaqMan™ Universal Master Mix II, with UNG

TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR).

Pricing and Availability
Specifications

GC-Rich PCR Performance	High
Content And Storage	Store at 2°C to 8°C in the dark. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Fidelity (vs. Taq)	2 X
Purity or Quality Grade	UP (Ultra Pure)
Concentration	2X
For Use With (Application)	Gene Expression,Genotyping,miRNA analysis
PCR Method	qPCR
Detection Method	Primer-probe
Polymerase	AmpliTaq Gold DNA Polymerase
Product Type	Universal Master Mix II
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7300 System, 7500 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Standard
Product Line	TaqMan™

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Applied Biosystems™ TaqMan™ GTXpress™ Master Mix

Combines AmpliTaq™ Fast DNA Polymerase, UP, dNTPs, Tracking Dye, and ROX™ dye in a convenient single vial format.

Pricing and Availability
Specifications

GC-Rich PCR Performance	High
Concentration	2X
For Use With (Application)	Genotyping
PCR Method	qPCR

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Applied Biosystems™ Fast SYBR™ Green Master Mix

Designed for speed, SYBR™ Green Master Mix provides a fast, reliable, and cost-effective solution for RT-PCR application without compromising sensitivity, specificity, dynamic range, or PCR efficiency.

Pricing and Availability
Specifications

GC-Rich PCR Performance	High
Purity or Quality Grade	UP (Ultra Pure)
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Detection Method	SYBR
Polymerase	AmpliTaq Fast DNA Polymerase, AmpliTaq Fast UP
Product Type	Real Time PCR SYBR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Fast
Label or Dye	SYBR Green
Product Line	SYBR™

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Applied Biosystems™ Power SYBR™ Green PCR Master Mix

Offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations.

Pricing and Availability
Specifications

GC-Rich PCR Performance	High
Purity or Quality Grade	LD (Low DNA)
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Detection Method	SYBR
Polymerase	AmpliTaq Gold DNA Polymerase, AmpliTaq Gold LD
Product Type	Real Time PCR SYBR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7300 System, 7500 System, 7700 System, 7900HT Fast System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Standard
Label or Dye	SYBR Green
Product Line	SYBR™

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Applied Biosystems™ TaqMan™ Fast Virus 1-Step Master Mix for qPCR

TaqMan Fast Virus 1-Step Master Mix is designed for fast, highly sensitive real-time RT-PCR, even in the presence of challenging PCR inhibitors.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
Reverse Transcriptase	M-MLV
Form	Liquid
InhibitorToleranceSimple	High
For Use With (Application)	Microbial Detection,Gene Expression,DNA Quantitation,Presence/Absence
PCR Method	1-step RT-qPCR
Multiplex Capability	Up to 3-plex
Detection Method	Primer-probe
Thermostability	72 hr., Assembled Reaction
Polymerase	AmpliTaq Fast DNA Polymerase
Format	Tube
Product Type	1-Step Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 7 Flex, QuantStudio™ Absolute Q Digital PCR System, StepOne™, StepOnePlus™, ViiA™ 7 System
Sample Type	Total RNA, Viral DNA, Viral RNA, mRNA
Reaction Speed	Fast or Standard
Target Specificity	RNA only, Both RNA and DNA
Hot Start	Built-In Hot Start
Product Line	TaqMan™

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Applied Biosystems™ SYBR™ Select Master Mix for CFX

A cost-effective solution for real-time PCR applications.

Pricing and Availability
Specifications

GC-Rich PCR Performance	High
Purity or Quality Grade	UP (Ultra Pure)
Form	Liquid
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Detection Method	SYBR
Polymerase	AmpliTaq DNA Polymerase
Format	Vial
Product Type	Real Time PCR SYBR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	AB 7900HT, Standard Mode, AB StepOne™, Fast Mode, AB 7500, Standard Mode, AB 7500, Fast Mode, Bio-Rad CFX96 Touch™, AB StepOnePlus™, Fast Mode, AB 7900HT, Fast Mode, AB StepOne™, Standard Mode, Bio-Rad CFX Connect™, AB 7900 HT 384-Well System, Bio-Rad CFX384 Touch™, AB StepOnePlus™, Standard Mode
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Fast or Standard
Label or Dye	SYBR GreenER
Hot Start	Built-In Hot Start
Product Line	SYBR™

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Applied Biosystems™ SYBR™ Select Master Mix

Cost-effective solution for real-time PCR applications.

Pricing and Availability
Specifications

GC-Rich PCR Performance	High
Content And Storage	The 2x mix contains SYBR™ GreenER™ dye, AmpliTaq™ DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye and optimized buffer components. Contains 1 X 1 mL tube, sufficient for 100, 20 μL reactions (10 μL Master Mix per reaction). Store at 2–8°C. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA). See user guide for primer concentration recommendations for the StepOne and StepOnePlus systems.
Purity or Quality Grade	UP (Ultra Pure)
Form	Liquid
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Detection Method	SYBR
Polymerase	AmpliTaq DNA Polymerase
Format	Vial
Product Type	Real Time PCR SYBR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Fast or Standard
Label or Dye	SYBR GreenER
Hot Start	Built-In Hot Start
Sufficient For	100 Reactions of 20 μL
Product Line	SYBR™

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Applied Biosystems™ TaqMan™ Fast Advanced Master Mix for qPCR

TaqMan Fast Advanced Master Mix delivers accurate quantification and dependability in less time on an array of qPCR instrument platforms.

Pricing and Availability
Specifications

Shipping Condition	Wet Ice
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	2 X
Form	Liquid
InhibitorToleranceSimple	High
Concentration	2X
For Use With (Application)	Gene Expression,DNA Quantitation,Presence/Absence,miRNA analysis
PCR Method	qPCR
Multiplex Capability	Duplex
Detection Method	Primer-probe
Includes	dUTP, UNG/UDG
Thermostability	72 hr., Assembled Reaction
Polymerase	AmpliTaq Fast DNA Polymerase
Product Type	Fast Advanced Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT System, QuantStudio Absolute Q Digital PCR System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 6 Pro, QuantStudio™ 7 Flex, QuantStudio™ 7 Pro, StepOne™, StepOnePlus™, ViiA™ 7 System
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Fast
Target Specificity	cDNA or gDNA only
Product Line	TaqMan™

Real Time PCR Master Mixes

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Applied Biosystems™ TaqPath™ qPCR Master Mix, CG