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  7. Invitrogen™ M-MLV Reverse Transcriptase (200 U/ÎĽL)
  8. 10338842

Invitrogen™ M-MLV Reverse Transcriptase (200 U/ÎĽL)

M-MLV Reverse Transcriptase is a recombinant DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid.

Brand:  Invitrogen™ 28025013

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Product Code. 10338842

  • 2760.00 NOK / Each
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Description

Description

M-MLV Reverse Transcriptase is a recombinant DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. Compared to AMV RT, Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) lacks DNA endonuclease activity and has a lower RNase H activity.

One unit of M-MLV RT is the amount of enzyme required to incorporate 1 nmole of deoxyribonucleotide into acid-precipitable material in 10 min at 37°C using poly(A) oligo(dT)25 as template-primer.

Features of M-MLV Reverse Transcriptase include:
• Excellent thermostability with optimal activity at 37°C
• Can be used to synthesize first-strand cDNA up to 7 kb
• Multiple applications including synthesis of first-strand cDNA, primer extension, sequencing dsDNA, cDNA libraries, and RT-PCR

Source
Purified from E. coli expressing the pol gene of M-MLV on a plasmid

Performance and quality testing
SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product

Unit reaction conditions
50 mM Tris-HCl (pH 8.3), 40 mM KCl, 6 mM MgCl2, 1 mM DTT, 0.5 mM [3H]dTTP, 0.1 mM poly(A), 0.1 mM oligo(dT)25, 0.1 mg/mL BSA, and enzyme in 50 μL for 10 min at 37°C

Order Info

Shipping Condition: Wet ice

TRUSTED_SUSTAINABILITY
Specifications

Specifications

200 U/ÎĽL
Stand-alone Enzyme
Reverse Transcription
40,000 units
Yes
First-Strand cDNA
Separate components
Up to 7 kb

• M-mLV RT, 200 μL (200 U/μL)
• 5X First Strand Buffer, 1 mL
• DTT, 500 μL (100 mM)

Store at –20°C.
50 min.
37°C
M-MLV
Wet Ice
200 Reactions
Reverse Transcription
RNA
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Lot Number3026183 Certificate TypeCertificate of Analysis Date26/12/2024 Catalog Number28025013
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Lot Number2740350 Certificate TypeCertificate of Analysis Date15/11/2023 Catalog Number28025013
Lot Number2676859 Certificate TypeCertificate of Analysis Date22/06/2023 Catalog Number28025013
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Product Title
Invitrogen™ M-MLV Reverse Transcriptase (200 U/ÎĽL) > 200 Reactions; 40,000 units

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For Research Use Only. Not for use in diagnostic procedures.