Protein Extraction and Purification

Thermo Scientific™ CHAPS Detergent (3-((3-cholamidopropyl) dimethylammonio)-1-propanesulfonate)

Non-denaturing Zwitterionic detergents that disrupt nonspecific protein interactions with less protein aggregation than nonionic detergents.

Thermo Scientific™ Pierce™ Disposable Columns, 5 mL

Empty drip columns (0.5 to 5 mL) with matching frits and caps for packing beaded affinity or desalting resins to use in gravity-flow purification techniques.

Gibco™ Expi293™ Expression System Kit

Designed with transient expression technology for rapid and ultra high-yield protein production from mammalian cells. Gibco™ Expi293™ Expression System Kit is based on high density culture of Expi293F™ cells in Expi293™ Expression Medium.

Thermo Scientific™ Triton™ X-100 Surfact-Amps™ Detergent Solution

Triton™ X-100 Surfact-Amps™ Detergent Solution

GE Healthcare Net Rings

Use with XK 16 Columns and C 16 Columns. GE Healthcare Net Rings are designed for Columns, i.d 16 mm, 10 µm

Thermo Scientific™ Pierce™ HA-Tag IP/Co-IP Kit

Anti-HA antibody agarose beads and complete reagent set to easily co-immunoprecipitate protein interactions involving HA-tagged fusion proteins.

Thermo Scientific™ RIPA Lysis and Extraction Buffer

Lyse cultured mammalian cells with this high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent.

Thermo Scientific™ Pierce Protease and Phosphatase Inhibitor Mini Tablets,EDTA-free

Thermo Scientific™ Pierce Protease and Phosphatase Inhibitor Mini Tablets broad-spectrum protease and pghospahatase inhibitor formulation provides complete protection from dephosphorylation and proteolytic degradation.

GE Healthcare Superdex™ 200 Increase 3.2/300 Prepacked Precision Column

Used for micro-scale (μg) preparative purification and for characterization and analysis of small volumes (4 to 50μL) of proteins.  GE Healthcare Lifescience™ Superdex™ 200 Increase 3.2/300 Prepacked Precision Column desigened for size exclusion chromatography.

Thermo Scientific™ Pierce™ Classic IP Kit

Perform traditional immunoprecipitation assays with ease using high-quality Protein A/G agarose beads, microcentrifuge spin columns and buffers.

Gibco™ Human Microsomes, 50 Donors

Human Microsomes, 50 Donors

Gibco™ Human S9 Fractions

Human S9 Fractions

Thermo Scientific™ HisPur™ Ni-NTA Spin Plates

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

Thermo Scientific™ Halt™ Protease Inhibitor Cocktail (100X)

Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors.

Thermo Scientific™ Sodium Dodecyl Sulfate (SDS), Lauryl

Perform routine protein electrophoresis and cell lysis methods using this popular, standard-grade SDS detergent.

Thermo Scientific™ Pierce™ Protease Inhibitor Mini Tablets

Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments

Thermo Scientific™ NAb™ Protein L Spin Column, 5mL

Affinity purify antibodies that have kappa light chains using Protein L offered in bottles, spin columns, filter plates and complete purification kits.

Merck Millipore Fast-Trap™ Adenovirus and Lentivirus Purification and Concentration Kits

Quick, easy membrane-based method for laboratory-scale virus purification

Merck Millipore Biomax™ Ultrafiltration Membrane Discs

Recommended for concentrating or desalting higher volumes of more concentrated samples such as serum, plasma, or conditioned tissue culture media. Biomax Ultrafiltration Membrane Discs are compatible with Amicon™ Stirred Cells.

GE Healthcare His GraviTrap™ Kit

For fast and simple purification of histidine-tagged proteins

GE Healthcare Sephadex™ G50 Medium Media

Suitable for gel filtration

Thermo Scientific™ Pierce™ Protein Refolding Kit

Refold recombinant proteins that have been denatured and solubilized from inclusion bodies by determining optimal buffer conditions using this reagent matrix strategy.

Invitrogen™ DynaMag™-15 Magnet

Optimized for efficient cell isolation using all types of Dynabeads™in standard 15mL lab tubes

Sodium Dodecyl Sulfate (SDS), White Powder, Electrophoresis, Fisher BioReagents™

CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.378 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M Synonym: Sodium Lauryl Sulfate, SDS PubChem CID: 3423265 ChEBI: CHEBI:8984 IUPAC Name: sodium;dodecyl sulfate SMILES: CCCCCCCCCCCCOS(=O)(=O)[O-].[Na+]

Thermo Scientific™ HisPur™ Cobalt Spin Columns, 3 mL

Gently purify His-tagged recombinant fusion proteins using cobalt (Co2+) immobilized metal chelate affinity (IMAC) beads, spin columns and plates.

Thermo Scientific™ pT7CFE1-CGFP-HA-His Vector for Mammalian Cell-Free Protein Expression

Generate His-, HA-, Myc- or other label-tagged fusion proteins by cell-free in vitro translation (IVT) with these versatile T7 expression vectors.

Invitrogen™ Exosome-Streptavidin Isolation/Detection Reagent

Quickly and easily purify pre-enriched CD63+ exosomes from cell culture media

Gibco™ Rat (Sprague Dawley) Microsomes

Rat (Sprague Dawley) Microsomes

Thermo Scientific™ Pierce™ Glutathione Chromatography Cartridges, 5 mL

Purify GST-tagged recombinant fusion proteins from cell lysates using glutathione (GSH) beaded agarose affinity purification resins, spin columns and plates.

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