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  5. Thermo Scientific™ B-PER™ Complete Bacterial Protein Extraction Reagent

Thermo Scientific™ B-PER™ Complete Bacterial Protein Extraction Reagent

Product Code. 15265743 Shop All Thermo Scientific Products
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Quantity:
250 mL
500 mL
Unit Size:
250mL
500mL
Product Code. Quantity unitSize
15265743 500 mL 500mL
15255743 250 mL 250mL
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Product Code. 15265743

Brand: Thermo Scientific™ 89822

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5487.20 NOK valid until 2026-06-30
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This item is not returnable. View return policy

Description

Includes

Reagent packages are available in Tris and phosphate buffer formulations; Kits contain Tris-based B-PER Reagent plus lysozyme and DNase I enzymes.

Efficient, gentle lysis and extraction of E.coli and other bacterial cells

  • An easy-to-use cell lysis reagent that effectively disrupts cells and solubilizes native or recombinant proteins without denaturation
  • All B-PER Reagents are compatible with downstream applications such as affinity chromatography (e.g., immobilized metal affinity chromatography, glutathione chromatography), SDS-PAGE, and protein quantification (e.g., Pierce BCA Protein Assay, Pierce 660nm Protein Assay)
  • This all-inclusive formulation combines the lysis reagent with lysozyme, and a universal nuclease to enable mild extraction from both gram-negative and gram-positive bacteria
  • Depending on the particular application protease inhibitors, salts, reducing agents, denaturants, and chelating agents may be added to the reagent.

Features of the B-PER Bacterial Protein Extraction Reagent:

  • Detergent in Tris buffer with Lysozyme and Universal Nuclease
  • Improved cell lysis and DNA digestion, thereby releasing soluble proteins and reducing viscosity to increase yields
  • Recovery of large molecular weight proteins (high MW proteins)
  • Cost-effective equivalent to Merck Millipore Bugbuster™ Master Mix
  • More effective than traditional sonication and typical homemade lysis buffers, many of which include detergents and components that interfere with downstream applications
TRUSTED_SUSTAINABILITY

Specifications

Formulation Proprietary detergent in 20mM Tris buffer, pH 7.5, containing lysozyme and universal nuclease
Quantity 500 mL
Product Type Bacterial Protein Extraction Reagent
Volume (Metric) 500 mL
Content And Storage Store at 4°C.
Product Line B-PER

Safety and Handling

compliance-icons
Product Identifier
  • BPER Complete Bacterial Protein Extraction Reagent
 Signal Word
  • Warning
 Hazard Category
  • Serious eye damage/eye irritation Category 2
 Hazard Statement
  • H319-Causes serious eye irritation.
 Precautionary Statement
  • P264-Wash hands thoroughly after handling.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P337+P313-If eye irritation persists: Get medical advice/attention.
 Supplemental information
  • MIXTURE LIST-Contains : Poly(oxy-1,2-ethanediyl), .alpha.-[4-(1,1,3,3-tetramethyl butyl)phenyl]-.omega.-hydroxy

For Research Use Only. Not for use in diagnostic procedures.

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