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Cytiva NAP™ Columns, NAP-25
Use for rapid and efficient purification of DNA and oligonucleotides (>10-mer) in less than 15 minutes by gravity flow. Cytiva Life Sciences™ illustra™ NAP™ Columns are disposable columns prepacked with Sephadex™ G-25 DNA Grade
Includes | Complete step-by-step protocol identifying the fixed volumes needed for equilibration, washing and sample elution |
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Product Type | NAP-25 |
For Use With (Application) | Rapid and efficient purification of DNA and Oligonucleotides |
Cytiva NAP™ Columns, NAP-10
Use for rapid and efficient purification of DNA and oligonucleotides (>10-mer) in less than 15 minutes by gravity flow. Cytiva Life Sciences™ illustra™ NAP™ Columns are disposable columns prepacked with Sephadex™ G-25 DNA Grade
Includes | Complete step-by-step protocol identifying the fixed volumes needed for equilibration, washing and sample elution |
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Product Type | NAP-10 |
For Use With (Application) | Rapid and efficient purification of DNA and Oligonucleotides |
Cytiva PuReTaq Ready-To-Go™ PCR Beads
Pre-mixed, pre-dispensed reaction beads optimized for performing standard PCR amplifications. Cytiva Lifescience™ illustra™ PuReTaq Ready-To-Go™ PCR Beads are stable at ambient temperature.
Quantity | 1 Kit |
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For Use With (Application) | Standard PCR |
Cytiva ExoProStar™ 1-step
Use for enzymatic PCR clean-up. Cytiva Lifescience™ Illustra ExoProStar™ 1-step with illustra Exonuclease I and Alkaline Phosphatase combined in one tube to remove unincorporated primers and dNTPs.
Description | Enzymatic PCR clean up technology with illustra Exonuclease I and Alkaline Phosphatase combined in one tube to remove unincorporated primers and dNTPs. For maximum convenience comes in a single tube. |
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Product Type | illustra ExoProStar 1-Step |
Isolation Technology | Enzymatic PCR Clean-up Technology |
Quantity | 1 Each |
Yield | 100% recovery of PCR product |
For Use With (Application) | Remove unincorporated primers and dNTPs |
Cytiva NAP™ Columns, NAP-5
Use for rapid and efficient purification of DNA and oligonucleotides (>10-mer) in less than 15 minutes by gravity flow. Cytiva Life Sciences™ illustra™ NAP™ Columns are disposable columns prepacked with Sephadex™ G-25 DNA Grade
Includes | Complete step-by-step protocol identifying the fixed volumes needed for equilibration, washing and sample elution |
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Product Type | NAP-5 |
For Use With (Application) | Rapid and efficient purification of DNA and Oligonucleotides |
Cytiva MicroSpin™ S-400 HR Columns
Use for rapid purification of PCR products (>200 bp) from unincorporated primers (<32-mers) and nucleotides. Cytiva Life Sciences™ illustra™ MicroSpin™ S-400 HR Columns use spin-column chromatography for quick results.
Cytiva MicroSpin™ G-25 Columns
Use for the rapid purification of DNA >10 base pairs in length in a wide range of applications. Cytiva Life Sciences™ llustra™ MicroSpin™ G-25 Columns are ideal for desalting, buffer exchange, and removal of unincorporated nucleotides from end-labeled oligonucleotides.
Cytiva Amersham™ ECL™ Blocking Agent
Use for blocking nitrocellulose and PVDF membranes. Cytiva Life Sciences™ Amersham™ ECL™ Blocking Agent blocks membranes to be used with ECL and ECL Plus™ Western blotting detection reagents.
Cytiva ProbeQuant™ G-50 Micro Columns
For rapid purification of labeled DNA (>20 bases) from unincorporated labeled nucleotides in a volume of 25 to 50μL. Cytiva Life Sciences™ ProbeQuant™ G-50 Micro Columns use spin-column chromatography.
Cytiva TempliPhi™ 100/500 Amplification Kit
Use amplified DNA directly for cycle sequencing without purification. Cytiva illustra TempliPhi DNA Amplification Kits efficiently prepare DNA sequencing templates from circular DNA.
Includes | 0.5mL Sample Buffer, 0.5mL Reaction Buffer, 20μL Enzyme mix and 50μL positive control pUC19 DNA |
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Content And Storage | The product can be stored for 2 months or less at -20°C. For storage longer than 2 months, store at -70°CThaw components on ice and maintain at 0–4°C during handling. DO NOT warm above 4°C prior to amplification |
Description | illustra TempliPhi DNA Amplification Kits efficiently prepare DNA sequencing templates from circular DNA. |
For Use With (Application) | Sequencing Template Amplification Kits and Reagents |
Test Time | 4-18 h depending on starting material |
Cytiva SeraSil-Mag Silica Coated Superparamagnetic Beads
SeraSil-Mag beads for nucleic acid isolation deliver high purity DNA extraction in NGS applications.
Content And Storage | Room Temperature |
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Description | Storage Solution: Water + 0.05% sodium azide |
Product Type | Silica coated, core-shell structure |
Shelf Life | 24 months at room temperature |
Concentration | 20 mg/mL in water (0.05% sodium azide) |
For Use With (Application) | Chaotropic salt chemistry and vortex mixing, roller mixing and sonication |
Cytiva GenomiPhi V2 DNA Amplification Kit
Amplify whole genomes with high efficiency and representation. Cytiva Life Sciences™ illustra™ GenomiPhi V2 DNA Amplification Kit provides whole genome amplification with yields of 4 to 7μg.
Content And Storage | Store the kit at -70°C.The enzyme mix must be stored at -70°C; all other components may be stored at -20°C. Thaw components on ice and maintain at 0°C to 4°C during handling. |
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Description | Illustra GenomiPhi V2 DNA Amplification Kit offers highly efficient and representative whole-genome amplification with 4 to 7 μg yield from nanogram amounts of DNA sample. |
Product Type | illustra GenomiPhi V2 DNA Amplification Kit |
Size | > 10 kb |
For Use With (Application) | Genomic DNA Amplification |
Test Time | 1.5-2 h |
Cytiva GFX™ PCR DNA and Gel Band Purification Kit
Purify and concentrate PCR products or DNA fragments ranging in size from 50 bp to 10 kb. Cytiva Life Sciences™ illustra™ GFX™ PCR DNA and Gel Band Purification Kit can be used to purify DNA from reaction volumes up to 100μL or agarose gel slices of up to 900mg.
Includes | GFX™ columns, collection tubes, color-coded bottles of capture Buffer, Wash Buffer, two Elution Buffers |
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Description | Designed for the rapid purification and concentration of PCR products or DNA fragments ranging in size from 50 bp to 10 kb. This kit can be used to purify DNA from reaction volumes up to 100 μl or agarose gel slices up to 900 mg. |
Product Type | illustra GFX PCR DNA and Gel Band Purification Kit |
Formulation | Tris HCl and sterile Water |
Sample Type | Agarose Gel Slices,DNA Solutions,Enzyme Reactions,PCR Mixtures |
Sample Volume | 50 bp-10 kb |
Yield | PCR 10 μl elution volume-65%, PCR 50 μl elution volume-82%, 300 mg agarose 10 μl elution volume-57%, 300 mg agarose 50 μl elution volume-91% |
For Use With (Application) | Isolation and concentration of DNA fragments (50bp to 10kb) from PCR mixtures, DNA-containing Agarose Gel bands, Enzyme-based DNA modifications and restriction digestions |
Cytiva Taq DNA Polymerase (cloned)
For PCR and other procedures requiring DNA polymerase activity at elevated temperatures
Content And Storage | 100mM Tris HCl, pH 9, 15mM MgCl2 and 500mM KCl. Store at -20°C |
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Purity or Quality Grade | Free from detectable nonspecific nuclease and Taq I restriction endonuclease activities. |
pH | 7.5 |
Concentration | 5000U/mL |
For Use With (Application) | Standard PCR |
Cytiva RAPD Analysis Beads
For performing DNA-profiling experiments using the randomly amplified polymorphic DNA (RAPD) technique
Format | Tube |
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Volume | 500 μL |
pH | 8.3 |
For Use With (Application) | Standard PCR |