ELISA Substrates and Reagents
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Thermo Scientificâ„¢ 1-Stepâ„¢ TMB ELISA Substrate Solutions
Thermo Scientificâ„¢ Pierce TMB ELISA Substrates detect horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370 nm and 652 nm) that changes to yellow (Amax = 450 nm) upon addition of a sulfuric or phosphoric acid stop solution.
| Substrate Properties | Chemical Substrate |
|---|---|
| Product Type | ELISA Substrate |
| Substrate Type | HRP (Horseradish Peroxidase) |
| Detection Method | Colorimetric |
Thermo Scientificâ„¢ PNPP Substrates
Thermo Scientificâ„¢ Pierce PNPP Substrates are powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications.
| Substrate Properties | Chemical Substrate |
|---|---|
| Product Type | ELISA Substrate |
| Substrate Type | Alkaline Phosphatase |
| Detection Method | Colorimetric |
Thermo Scientificâ„¢ ELISA Carbonate Coating Buffer
For use as a plate coating buffer for Invitrogen Antibody Pairs or when developing a sandwich ELISA.
Invitrogenâ„¢ ELISA Stop Solution
A ready-to-use solution intended for use as a stop solution in ELISA applications that use horseradish peroxidase and TMB substrate solution.
| Shipping Condition | Ambient |
|---|---|
| Content And Storage | Store at 2°C to 8°C |
| Form | Liquid |
| Product Type | ELISA Stop Solution |
| Substrate Type | HRP, TMB |
Thermo Scientificâ„¢ QuantaBluâ„¢ Fluorogenic Peroxidase Substrate Kit
Conduct stopped, non-stopped and kinetic assays using this soluble fluorogenic (chemifluorescent) peroxidase substrate for ELISA.
Thermo Scientificâ„¢ 1-Stepâ„¢ ABTS Substrate Solution
Ready-to-use, water-soluble peroxidase substrate tablets that yield a measurable green end-product for use in ELISA methods.
Thermo Scientificâ„¢ Stop Solution for TMB Substrates
Stop TMB-based ELISA detection with this 0.16M sulfuric acid for use with the ELISA substrate 3,3',5,5' - tetramethylbenzidine (TMB).
BD OptEIAâ„¢ Human IL-15 ELISA Set
For developing ELISA for natural or recombinant human IL-15 in serum, plasma and cell culture supernatants
Thermo Scientificâ„¢ Pierceâ„¢ Diethanolamine Substrate Buffer (5X)
Optimized, reliable and convenient stock solutions of peroxide for HRP-based assays and diethanolamine for alkaline phosphatase (alk-phos) detection.
R&D Systemsâ„¢ Human TSLP DuoSet ELISA
Human TSLP DuoSet ELISA from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.
| Content And Storage | Store the unopened product at 2°C to 8°C. Do not use past expiration date. |
|---|---|
| Conjugate | Biotin |
| Product Type | ELISA Kit |
| Quantity | 1 Kit |
| Assay Range | 31.20 to 2000 pg/mL |
| For Use With (Application) | ELISA |
| Cross Reactivity | <50% cross-species reactivity observed with species tested. <0.5% cross-reactivity observed with available related molecules |
| Species | Human |
Molecular Probesâ„¢ DynaLightâ„¢ Substrate with RapidGlowâ„¢ Enhancer
DynaLightâ„¢ Substrate with RapidGlowâ„¢ Enhancer
Thermo Scientificâ„¢ ELISA Blocker Blocking Buffer
Increases signal-to-noise ratio and minimizes background
BD TMB Substrate Reagent Set
For use in BD OptEIAâ„¢ ELISA sets. Substrate Reagent A, which is clear and colorless, contains hydrogen peroxide. Substrate Reagent B is colorless to a very light amber solution containing 3,3', 5,5' tetramethylbenzidine (TMB) in an organic solvent.
Thermo Scientificâ„¢ SuperSignalâ„¢ ELISA Femto Substrate
HRP substrate formulated for superior protein detection and low-end linearity in chemiluminescent ELISA applications.
| Content And Storage | Stable for one year at 4°C or six months at room temperature. Product is shipped at ambient temperature. |
|---|---|
| Substrate Properties | Chemical Substrate |
| Product Type | ELISA Substrate |
| Substrate Type | HRP (Horseradish Peroxidase) |
| Product Line | SuperSignalâ„¢ |
| Detection Method | Chemiluminescence |