Immunoassay Reagents and Kits

Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.

We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.

ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.

Life Science Research ELISA Kits (15,413)

Singleplex and Multiplex Bead Based Immunoassays (1,416)

ELISA Substrates and Reagents (1,248)

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1 – 15 of 14,804 results

Invitrogen™ Human IL-8 ELISA Kit

ELISA

Pricing and Availability
Specifications

Target Species	Human
Conjugate	HRP
Interassay CV	6.1%
Intraassay CV	3.9%
Gene Symbol	CXCL8
Total Assay Time	2 hr 30 min
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	<5 pg/mL
Storage Requirements	2-8°C
Assay Range	15.6-1000 pg/mL
For Use With (Application)	Colorimetric
Test Time	1 hr 20 min
Accession Number	P10145
Regulatory Status	RUO
Target	IL-8
Kit Contents	Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
Precision	<5 pg/mL
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	3576
Sample Type	Plasma,Serum,Supernatant
Label or Dye	Biotin

Invitrogen™ Human Cortisol Competitive ELISA Kit

ELISA

Pricing and Availability
Specifications

Target Species	Human
Conjugate	HRP
Description	Cortisol Competitive ELISA Kit
Interassay CV	8.1%
Intraassay CV	8.8%
Total Assay Time	1 hr 30 min
Sample Volume	Fecal Extract 50 μL, Plasma 1 μL, Saliva 12.5 μL, Serum 1 μL, Supernatant 50 μL, Urine 6.25 μL
Assay Sensitivity	17.3 pg/mL
Storage Requirements	-20°C
Research Discipline	Inflammation
Assay Range	100-3200 pg/mL
For Use With (Application)	ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti
Test Time	1 hr 20 min
Detection Method	Fluorescence
Regulatory Status	RUO
Target	Cortisol
Kit Contents	Pre-coated 96 well plate, Standard, Assay Diluent concentrate, HRP-Conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,HRP-Conjugated Detection Antibody,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Sample Type	Fecal Extract,Plasma,Saliva,Serum,Supernatant,Urine
Label or Dye	HRP

Invitrogen™ Cytokine 25-Plex Human Panel

Specifically designed for quantifying human cytokines and chemokines in serum, plasma, and tissue culture supernatant

Pricing and Availability

Gibco™ Residual Protein TrypLE™ ELISA Kit

The Residual Protein TrypLE ELISA Kit is a solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative detection of TrypLE enzyme.

Pricing and Availability
Specifications

Shipping Condition	Wet Ice
Protein Aliases	TrypLE, R-Protease
Immunoassay Kit Format	Sandwich ELISA Kit
Content And Storage	• Pre-coated 96-well plate • Standard assay buffer concentrate • HRP-conjugated detection antibody • Wash buffer concentrate • Chromogen stop solution • Adhesive plate covers Store at 2–8°C.
Conjugate	Horseradish Peroxidase (HRP)
Interassay CV	6.20%
Intraassay CV	7.20%
Total Assay Time	1.5 hr.
Assay Sensitivity	0.032 ng/mL
Assay Range	0.78 to 50 ng/mL
Target	TrypLE (R-Protease) concentration
Format	Wash steps required
For Use With (Equipment)	Colorimetric Microplate Reader
Label or Dye	Horseradish Peroxidase (HRP)

Thermo Scientific™ 1-Step™ TMB ELISA Substrate Solutions

Thermo Scientific™ Pierce TMB ELISA Substrates detect horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370 nm and 652 nm) that changes to yellow (Amax = 450 nm) upon addition of a sulfuric or phosphoric acid stop solution.

Pricing and Availability
Specifications

Substrate Properties	Chemical Substrate
Product Type	ELISA Substrate
Substrate Type	HRP (Horseradish Peroxidase)
Detection Method	Colorimetric

Invitrogen™ Superoxide Dismutase (SOD) Colorimetric Activity Kit

Assay Superoxide Dismutase (SOD) Activity in Serum, Plasma, Cells, Tissues and Erythrocyte Lysates.

Pricing and Availability
Specifications

Shipping Condition	Approved for shipment on Wet or Dry Ice
Immunoassay Kit Format	Assay Activity Kit
Content And Storage	96-well plate(s), standard, detection reagent, assay buffer, substrate, and lot-specific technical data sheet. Store at –20°C.
For Use With (Equipment)	Microplate Reader
Target Kit Named	Superoxide Dismutase (SOD)

Thermo Scientific™ QuantaBlu™ Fluorogenic Peroxidase Substrate Kit

Conduct stopped, non-stopped and kinetic assays using this soluble fluorogenic (chemifluorescent) peroxidase substrate for ELISA.

Pricing and Availability

Thermo Scientific™ PNPP Substrates

Thermo Scientific™ Pierce PNPP Substrates are powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications.

Pricing and Availability
Specifications

Substrate Properties	Chemical Substrate
Product Type	ELISA Substrate
Substrate Type	Alkaline Phosphatase
Detection Method	Colorimetric

Invitrogen™ Cell Lysis Buffer II

Cell Lysis Buffer II is a high-quality, ready-to-use lysis buffer suitable for the preparation of cell extracts for ELISA, western blotting, and antibody bead immunoassays (Luminex) applications.

Pricing and Availability

Thermo Scientific™ SuperSignal™ ELISA Pico Chemiluminescent Substrate

HRP substrate optimized to generate an intense light signal and provide exception performance in luminometer-based assays.

Pricing and Availability
Specifications

Content And Storage	Upon receipt store product at room temperature. Product shipped at ambient temperature.Introduction
Substrate Properties	Chemical Substrate
Product Type	ELISA Substrate
Substrate Type	HRP (Horseradish Peroxidase)
Product Line	SuperSignal
Detection Method	Chemiluminescence

Invitrogen™ Human IP-10 (CXCL10) ELISA Kit

ELISA

Pricing and Availability

Human IL-8 ELISA Development Kit (ABTS), PeproTech®

ELISA

Pricing and Availability

Invitrogen™ Human MMP2 ELISA Kit

ELISA

Pricing and Availability
Specifications

Target Species	Human
Conjugate	HRP
Interassay CV	5.6%
Intraassay CV	3.3%
Incubation Time	4 hr.
Gene Symbol	MMP2
Total Assay Time	4 hr
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	<1 ng/mL
Storage Requirements	2-8°C
Assay Range	0.78-50 ng/mL
Marker Gene	MMP-2
For Use With (Application)	Colorimetric Detection
Test Time	1 hr 20 min
Accession Number	P08253
Regulatory Status	RUO
Target	MMP2
Kit Contents	Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	4313
Sample Type	Plasma,Serum,Supernatant
Label or Dye	Biotin

Invitrogen™ Human NF-L ELISA Kit

ELISA

Pricing and Availability
Specifications

Shipping Condition	Wet Ice
Immunoassay Kit Format	Sandwich ELISA Kit
Target Species	Human,Mouse,Rat
Conjugate	HRP
Interassay CV	4%
Intraassay CV	3.7%
Gene Symbol	NEFL
Total Assay Time	3 hr 45 min
Assay Sensitivity	0.8 pg/mL
Storage Requirements	2-8°C
Assay Range	1.6-1200
Test Time	1 hr 20 min
Accession Number	P07196, P08551, P19527
Regulatory Status	RUO
Target	NF-L
Kit Contents	Pre-coated microwell plate, Standard, Biotinylated Detection Antibody, Streptavidin-HRP, Sample Diluent, Wash Buffer Concentrate, Assay Buffer concentrate, Stop Solution, Substrate Solution, Adhesive Plate Covers
Gene Alias	68 kDa neurofilament protein; AI847934; CMT1F; CMT2E; FLJ53642; light molecular weight neurofilament protein; micro glutamic acid-rich protein; NEF3; NEFL; nefl.L; neurofilament; neurofilament 3; neurofilament L subunit; neurofilament light; neurofilament light polypeptide; neurofilament protein; neurofilament protein L; neurofilament protein, light chain; neurofilament subunit NF-L; neurofilament triplet L protein; neurofilament, light L homeolog; neurofilament, light polypeptide; neurofilament, light polypeptide 68kDa; neurofilament, light polypeptide L homeolog; neurofilament-l; neurofilament-light; Nf68; NFL; N-FL; NF-L; NFM; NF-M; NLC; OTTHUMP00000196614; PPP1R110; protein phosphatase 1, regulatory subunit 110; XELAEV_18018673mg; XNF-L68 kDa neurofilament protein; AI847934; CMT1F; CMT2E; FLJ53642; light molecular weight neurofilament protein; micro glutamic acid-rich protein; NEF3; NEFL; nefl.L; neurofilament; neurofilament 3; neurofilament L subunit; neurofilament light; neurofilament light polypeptide; neurofilament protein; neurofilament protein L; neurofilament protein, light chain; neurofilament subunit NF-L; neurofilament triplet L protein; neurofilament, light L homeolog; neurofilament, light polypeptide; neurofilament, light polypeptide 68kDa; neurofilament, light polypeptide L homeolog; neurofilament-l; neurofilament-light; Nf68; NFL; N-FL; NF-L; NFM; NF-M; NLC; OTTHUMP00000196614; PPP1R110; protein phosphatase 1, regulatory subunit 110; XELAEV_18018673mg; XNF-L;
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	18039, 4747, 83613
Sample Type	Cerebrospinal Fluid,Plasma,Serum,Supernatant
Label or Dye	Biotin

Invitrogen™ Human IGF-1 ELISA Kit

ELISA

Pricing and Availability
Specifications

Conjugate	HRP
Interassay CV	<12%
Intraassay CV	<10%
Gene Symbol	IGF1
Total Assay Time	4 hr 45 min
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	0.1 ng/mL
Storage Requirements	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Assay Range	0.1-30 ng/mL
Test Time	1 hr 20 min
Accession Number	P05019
Regulatory Status	RUO
Target	IGF1
Kit Contents	Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	3479
Sample Type	Plasma,Serum,Supernatant
Label or Dye	Biotin

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