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  5. Thermo Scientific™ SuperSignal™ ELISA Pico Chemiluminescent Substrate

Thermo Scientific™ SuperSignal™ ELISA Pico Chemiluminescent Substrate

Product Code. 10475775 Shop All Thermo Scientific Products
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Quantity:
100 mL Kit
250 mL Kit
Kit Contents:
Stable Peroxide Buffer, 125mL, Luminol/Enhancer, 125mL
Stable Peroxide Buffer, 50mL, Luminol/Enhancer, 50mL
Unit Size:
100mL
250mL
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Product Code. Quantity Kit Contents unitSize
10475775 250 mL Kit Stable Peroxide Buffer, 125mL, Luminol/Enhancer, 125mL 250mL
10185663 100 mL Kit Stable Peroxide Buffer, 50mL, Luminol/Enhancer, 50mL 100mL
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Product Code. 10475775 Supplier Thermo Scientific™ Supplier No. 37069

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Description

HRP substrate optimized to generate an intense light signal and provide exception performance in luminometer-based assays.

Thermo Scientific™ SuperSignal ELISA Pico Chemiluminescent Substrate is optimized to generate an intense light signal and provide exception performance in luminometer-based assays.

SuperSignal ELISA Pico Substrate provides chemiluminescent detection of horseradish peroxidase (HRP) activity for ELISA methods based on HRP-conjugated antibodies and probes. These ELISAs can take place in either a test tube or a microplate and are quantified by measuring relative light units (RLU) in a luminometer.

Highlights:

  • Immediate light generation – intense signal is produced immediately at room temperature or at 37°C
  • High signal-to-noise ratio – minimal background
  • Low picogram sensitivity – detect proteins in your ELISAs down to the picogram levels
  • Room temperature storage – a consistent product with ambient shipping and no need to store at 4°C
  • 8-hour working solution stability – consistent performance of the working solution over an 8-hour period with only a 10% decrease in activity at 24 hours
  • Flexible – signal can be read in black or white opaque plates
  • Emits light at 425nm – measurable by luminometers

Includes:

Luminol/Enhancer Solution (125 mL); Stable Peroxide Buffer (125 mL)

TRUSTED_SUSTAINABILITY

Specifications

Product Type ELISA Substrate
Quantity 250 mL Kit
Detection Method Chemiluminescence
Product Line SuperSignal
Substrate Properties Chemical Substrate
Substrate Type HRP (Horseradish Peroxidase)
Kit Contents Stable Peroxide Buffer, 125mL, Luminol/Enhancer, 125mL
Content And Storage Upon receipt store product at room temperature. Product shipped at ambient temperature.Introduction

Frequently Asked Questions (FAQs)

What type of instrument do I use to read the plates detected with the SuperSignal ELISA Pico Chemiluminescent Substrate?

A luminometer is used to measure relative light units.
What microplates work best with the SuperSignal ELISA Pico Chemiluminescent Substrate?

White plates provide the best signal-to-noise ratio. Black plates provide lower background but less detectable signal.
What signal duration occurs with SuperSignal ELISA Pico Chemiluminescent Substrate?

Maximum signal is reached within five minutes and then declines slowly thereafter until it is much less after an hour.
How sensitive is SuperSignal ELISA Pico Chemiluminescent Substrate?

The substrate allows measurement of low picogram levels in sandwich ELISA procedures.
How does the SuperSignal ELISA Pico Chemiluminescent Substrate compare with QuantaBlu Fluorogenic Peroxidase Substrate?

QuantaBlu Fluorogenic Peroxidase Substrate is a fluorescent detection reagent for HRP and provides attamole sensitivity. SuperSignal ELISA Pico Chemiluminescent Substrate is strictly chemiluminescent.
Can I dilute the SuperSignal ELISA Pico Chemiluminescent Substrate working reagent if my signal is too intense?

We do not recommend diluting the working reagent. It is better to dilute or otherwise optimize primary and or secondary antibody concentrations or use more washes between incubation steps.
At what wavelength is SuperSignal ELISA Pico Chemiluminescent Substrate signal measured?

The light emission wavelength is read at 425 nm.
When using the SuperSignal ELISA Pico Chemiluminescent Substrate, why are the wells appearing red-orange-yellow in relation to the enzyme?

The substrate solution should remain colorless upon reaction with Horseradish peroxidase (HRP), if the HRP concentration is within the appropriate range. Extremely high concentrations of HRP can cause the substrate color to turn yellow/orange/brown. Alternatively, the color change of the substrate may be due to the presence of some other contaminant.

For Research Use Only. Not for use in diagnostic procedures.

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