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  5. Invitrogen™ Bovine TNF alpha ELISA Kit

Invitrogen™ Bovine TNF alpha ELISA Kit

Product Code. p-7230649
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Quantity:
5 x 96 Tests
96 Tests
Conditionnement:
1 set
Each
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Code produit. 15454873

Marque: Invitrogen™ EBTNF

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Description

ELISA

The Bovine Tumor Necrosis Factor alpha (Bov TNFα) ELISA quantitates Bov TNFα in bovine serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Bov TNFα. Principle of the method The Bovine TNFα solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.
TRUSTED_SUSTAINABILITY

Spécification

Accession Number Q06599
Assay Range 0.123 to 30 ng/mL
Assay Sensitivity 0.1 ng/mL
Calibration Range 0.1 ng/ml to 30 ng/ml
Description TNF-a (TNF) ELISA Kit, 96 Assays, 0.1 to 30ng/mL Test Range, 5 hr. Test Time, 0.1ng/mL Sensitivity, Cell Culture Supernatants, Plasma, Serum Sample, Cattle/Bovine Species, Colorimetric Detection Method, TNF Gene, Used for Protein Assays and Analysis, 2 to
Detectable Analytes TNF alpha
Assay Solid-phase sandwich ELISA
Conjugate HRP
Detection Method Colorimetric
Product Type ELISA
Protein Family Kinase & Inhibitors
Sample Type Plasma, Serum, Supernatant
Specificity This ELISA antibody pair detects bovine TNF-alpha. Other species not determined
Sufficient For 96 Assays
Target Species Bovine
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 280943
Gene Symbol TNF-a,TNF-alpha,TNFa
Incubation Time 5 hr.
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Research Discipline Extracellular Matrix & Cell Adhesion
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Storage Requirements 2°C to 8°C
Synonym BOS
Target TNF alpha
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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