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Description
The nearly irreversible binding is validated for fusion proteins carrying a C- or N-terminal Strep-tag™II providing an N-terminal extension by a SerAla linker (recombinant protein-SA-WSHPQFEK or SA-WSHPQFEK-recombinant protein).
Proteins containing Strep-tag™II with N-terminal sequences other than SerAla are bound with reduced affinity in the cases tested so far, the Twin-Strep-tag™ always contains the SerAla linker (WSHPQFEK-GGGS-GGGS-GGSA-WSHPQFEK). StrepMAB-Immo is available as conjugate with different fluorescent dyes (DY-488, DY-549, DY-649).
The absorption/emission maxima of the fluorescent dyes are:
• DY-488: 493/518 nm
• DY-549: 550-560/575 nm
• DY-649: 655/676 nm
Please note: For detection of Strep-tag™II or Twin-Strep-tag™ fusion proteins in western blots, the use of StrepMAB-Classic or Strep-Tactin™ is recommended.
Specifications
Specifications
| Capacity (Metric) | 0.10 mL |
| Concentration | 0.5 mg/mL |
| Kit Contents | Solution in PBS; 0.01 % NaN3; 1 mg/ mL BSA |
| Content And Storage | Undiluted: 2°C to 8°C, protect from light; Diluted: -25°C to -15°C |
| Quantity | 50 μg |
| For Use With (Application) | ELISA, FACS, immunofluorescence |
| Target | Strep-tag™II and Twin-Strep-tag™ |
| Type | Conjugate |
| Label or Dye | DY-488 |
For Research Use Only.
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