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  6. IBA Lifesciences Strep-Tactin™ 4Flow™ high capacity FPLC column

IBA Lifesciences Strep-Tactin™ 4Flow™ high capacity FPLC column

Product Code. 30117607 Shop All IBA Lifesciences Products
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Capacity (Metric):
1 mL
5 mL
Unit Size:
1mL
5mL
Product Code. Capacity (Metric) unitSize
30117607 5 mL 5mL
30117608 1 mL 1mL
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Product Code. 30117607

Brand: IBA Lifesciences 21258001

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Description

Strep-Tactin™ 4Flow™ high capacity FPLC column to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins

  • Strep-Tactin™ 4Flow™ high capacity FPLC columns are designed for purifying Strep-tag™II and Twin-Strep-tag™ proteins via affinity chromatography in automated protein purification with HPLC/FPLC devices such as Äkta™ systems (Cytiva).
  • Recommended for all protein classes due to the broad pH compatibility, excellent pressure stability, outstanding target purity (>95%), and high binding affinity
  • 4% crosslinked agarose beads coupled to Strep-Tactin™ for straightforward purification of proteins with Strep-tag™II or a Twin-Strep-tag™
  • Multiple regeneration cycles possible without loss of binding capacity
  • Ideal alternative for proteins that are difficult to elute from Strep-Tactin™XT 4Flow™ resins
  • Biotinylated proteins also bind to Strep-Tactin™ 4Flow™ high capacity and need to be blocked using BioLock

Specifications

Binding Capacity 20 mg/mL resin
Capacity (Metric) 5 mL
Description 4% agarose, crosslinked
Particle Size 50 to 150 μm
Form Resin Slurry
Quantity 5 mL
Protein Form Tetrameric protein
Target Proteins tagged with Twin-Strep-tag™; Strep-tag™II; cross reactivity: biotin
Type Column
Final Product Type Affinity chromatography equipment
Ligand Type Twin-Strep-tag™; Strep-tag™II; cross reactivity: biotin
Stationary Phase Agarose
Format FPLC cloumn
Column Type Pre-packed gravity flow column
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