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Macherey-Nagel™ Protino Ni-NTA Agarose for His-tag Protein Purification
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Quantity:
25 mL
500 mL
Packungsgröße:
25mL
500mL
Beschreibung
- Enable fast and convenient purification of recombinant polyhistidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
- Proteins from any expression system can be purified under native or denaturing conditions.
- Binding of protein is based on the interaction between the polyhistidine tag of the recombinant protein and immobilized Ni2+ ions.
- Consists of the chelating ligand nitrilotriacetic acid (NTA) immobilized on 6% cross-linked agarose beads that are suitable for batch binding, gravity flow, and FPLC™ columns.
- The resin is precharged with Ni2+ ions and therefore ready to use.
- Technology: Immobilized metal ion affinity chromatography
- Batch binding, gravity flow column chromatography, MPLC / FPLC applications
- Matrix: 6 % beaded agarose (cross-linked), precharged with Ni2+
- Bead size: 45 to 165 μm
Spezifikation
Spezifikation
| Binding Capacity | <50 mg/mL settled agarose |
| Description | Aqueous suspension |
| Content And Storage | 4°C to 8°C |
| Quantity | 25 mL |
| For Use With (Application) | Purification of His-tag proteins |
| Target | Protein |
| Type | Agarose |
| Ligand Type | NTA (nitrilotriacetic acid) |
Sicherheit und Handhabung
Signalwort
- Warning
- Flammable liquid Category 3
- Skin sensitiser Category 1
- H226-Flammable liquid and vapour.
- H317-May cause an allergic skin reaction.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- MIXTURE LIST-Contains : ethanol (diluted < 20 %) (denatured with1%IPA/1%MEK, acc.2016/1867/EU), resin suspension with Ni in
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