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Description
Thermo Scientific™ Pierce Renilla Luciferase Glow Assay Kit provides an extremely bright and stable bioluminescence signal (half-life approx. 3 hours), especially in the presence of Green Renilla luciferase reporter.
This Pierce Luciferase Glow Assay Kit contains reagents for measuring the activity of Renilla luciferase in cell lysates. In the presence of luciferase, the glow reagent provides stable bioluminescent signal and is highly suited for luminometers without injectors or for batch processing of samples. The light output generated by the luciferase reaction can be correlated with the amount of luciferase protein produced which in turn is proportional to the promoter activity driving the luciferase expression. The glow assay reagents were optimized for best results with Thermo Scientific Green Renilla Luc Plasmids; however, this glow kit may be used to detect the activity of other Renilla luciferase derivatives that use coelenterazine as a substrate.
Highlights
- Sensitive—highly sensitive detection of Green Renilla luciferase activity
- Stable—increased signal stability compared to flash assays
- Simple—does not require a luminometer with injectors
- Compatible—assay reagents compatible with other Renilla luciferases
- Automation-friendly—amenable to high throughput assays
- Convenient—contains a universal cell lysis buffer and optimized glow assay reagent
- Safe—allows one to perform non-radioactive assays
Requires
Renilla luciferase and luminometer or other instrument capable of monitoring luminescence, such as the Varioskan ALF or the Varioskan LUX microplate readers
Recommended applications
Promoter studies for analyzing cis- regulatory elements and trans-acting factors; Drug screening; siRNA and miRNA screening; Multiplexed assays to study off-target effects; Protein localization reporter assays; Signal transduction pathway analysis; RNA splicing studies
Specifications
Specifications
| Type | Assay Kit |
| Target | Luciferase, Green Renilla Luciferase |
| Quantity | 100 Reaction Kit |
| Content And Storage | Upon receipt store kit at -80°C or store individual components as indicated above. • Renilla Glow Assay Buffer, 5 mL, store at 4°C • Coelenterazine (100X), 50 μL, store at -80°C • 2X Cell Lysis Buffer, 6 mL, store at room temperature |
| Assay | Reporter Enzyme, Luciferase Reporter Assay |
| Compatible Cells | Mammalian Cells |
| Detection Method | Bioluminescence |
| For Use With (Equipment) | Luminometer (Microplate) |
| Format | 384-well plate, 96-well plate |
| Label Type | Enzyme Labeled |
| Show More |
Safety and Handling
- Pierce Renilla Luciferase Glow Assay Kit
- Danger
- Acute toxicity Category 3
- Flammable liquid Category 2
- Specific target organ toxicity after single exposure Category 1
- H225-Highly flammable liquid and vapour.
- H301-Toxic if swallowed.
- H311-Toxic in contact with skin.
- H331-Toxic if inhaled.
- H370-Causes damage to organs.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P405-Store locked up.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contains : methanol
For Research Use Only. Not for use in diagnostic procedures.
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