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Pierce™ Pierce™ Ni-NTA Magnetic Agarose Beads

Product Code. 15656144 Shop All Thermo Scientific Products
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Product Code. 15656144 Supplier Pierce™ Supplier No. 78605
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Beskrivning

Thermo Scientific™ Pierce™ Ni-NTA Magnetic Agarose Beads provide a fast, convenient method for purification of His-tagged recombinant proteins.

Product features:

Ni-NTA Magnetic Agarose Beads are used for small-scale affinity purification as well as high-throughput screening of recombinant His-tagged proteins. The polyhistidine tag is the most popular affinity tag and typically consists of six consecutive histidine residues (6xHis). Tagged proteins are overexpressed in a number of different systems, most commonly in bacteria, and purified from cell lysates such as those prepared using Thermo Scientific B-PER Bacterial Protein Extraction reagents. Purification of His-tagged proteins is achieved using an NTA chelate charged with nickel that coordinates with the histidine side chains. The NTA chelate contains four metal-binding sites that allow for low metal ion leaching and high binding capacity. The protocol for the Ni-NTA Magnetic Agarose Beads has been optimized to allow for high purity of the isolated His-tagged protein.

  • High capacity—sufficient for both routine and demanding purification procedures; binds >70 mg of 6xHis-tagged protein per mL of settled resin
  • High-performance and reproducible beads—non-aggregating, magnetite (Fe3O4), superparamagnetic beads provide exceptional uniformity for both manual and automated HTS purification applications
  • Low nonspecific binding—optimized purification protocol results in better tagged-protein purification
  • Versatile—purify proteins using native or denaturing conditions
  • Compatible—use with Thermo Scientific Cell Lysis reagents and a variety of buffer additives

Applications:

  • Protein purification and isolation
  • High throughput protein purification using automated magnetic particle processor
TRUSTED_SUSTAINABILITY

Specifikationer

Bed Volume 1 mL
Capacity (Metric) 4 mL
Concentration Slurry: 25%
Content And Storage 1 mL settled resin. Supplied as 4 mL of 25% v/v suspension in 20% ethanol.

Store at 2–8°C.
Shipping Condition Wet Ice
Particle Size 30 μm
Form Liquid Suspension
Product Line Pierce
Quantity 4 mL
Protein Form Recombinant
Type Magnetic Agarose Bead
Stationary Phase Metal Chelate, Divalent Nickel
Format Bottle
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For Research Use Only. Not for use in diagnostic procedures.

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