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Thermo Scientific™ Mitochondria Isolation Kit for Tissue

Product Code. 10521294 Shop All Thermo Scientific Products
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Quantity:
115 mL
Unit Size:
1 set
Product Code. Quantity unitSize
10521294 115 mL 1 set
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Product Code. 10521294

Brand: Thermo Scientific™ 89801

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Description

Isolate intact mitochondria from soft and hard tissue samples in about an hour with this versatile, benchtop microcentrifuge-based fractionation kit.

Isolate intact mitochondria from soft and hard tissue samples in about an hour with Thermo Scientific™ Mitochondria Isolation Kit for Tissue.

The kit provides for two methods for mitochondria isolation. The first method uses a unique reagent-based procedure that enables simultaneous multi-sample processing. The second method relies on traditional Dounce homogenization for tissue disruption and subsequent isolation of the organelle. Both procedures use differential centrifugation to separate the intact mitochondria using a bench-top microcentrifuge and are completed in less than 60 minutes. In addition, both procedures have been optimized for maximum yield of mitochondria with minimal damage to integrity. The isolated mitochondria may be used for a number of downstream applications, including 1D and 2D Western blotting and protein profiling using mass spectroscopy.

Highlights:

  • Quick and convenient – isolate intact mitochondria in less than 60 minutes

  • Versatile – offers two methods of isolation from both soft- and hard-tissue samples

  • Multi-sample format – reagent-based approach enables simultaneous processing of multiple samples

  • Optional alternate method – reagents and protocol included for the traditional Dounce homogenization procedure with fewer required strokes

  • Benchtop-compatible – both procedures are performed using a microcentrifuge tube

Includes:

Mitochondria Isolation Reagents A, B and C; bovine serum albumin and a phosphate-buffered saline pack

TRUSTED_SUSTAINABILITY

Specifications

Product Type Cell Isolation Kit
Content And Storage Upon receipt store kit at 4°C. Product is shipped at ambient temperature.
Form Liquid
Reagent Type Organelle Isolation, Subcellular Fractionation
Sufficient For 50 Isolations
Quantity 115 mL

Safety and Handling

compliance-icons
Product Identifier
  • Mitochondria Isolation Kit for Tissue
 Signal Word
  • Warning
 Hazard Category
  • Serious eye damage/eye irritation Category 2
 Hazard Statement
  • H319-Causes serious eye irritation.
 Precautionary Statement
  • P264-Wash hands thoroughly after handling.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
 Supplemental information
  • MIXTURE LIST-Contains : Sodium chloride

For Research Use Only. Not for use in diagnostic procedures.

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