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  6. Thermo Scientific™ Pierce™ Microdialysis Plates

Thermo Scientific™ Pierce™ Microdialysis Plates

Product Code. 17332240 Shop All Thermo Scientific Products
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MWCO:
10 kDa
2 kDa
20 kDa
3.5 kDa
Not Applicable
Capacity (Metric):
0.1 mL
0.3 mL
1 mL
2.2 mL
Product Type:
Deep-well Plate, 96-well
Microdialysis Plate, 48-well
Microdialysis Plate, 96-well
Unit Size:
1 set
Each
Pack of 12
13 product options available for selection
Product selection table with 13 available options. Use arrow keys to navigate and Enter or Space to select.
Product Code. MWCO Capacity (Metric) Product Type unitSize
17332240 10 kDa 1 mL Microdialysis Plate, 48-well Each
11804564 10 kDa 0.1 mL Microdialysis Plate, 96-well Pack of 12
11502731 Not Applicable 2.2 mL Deep-well Plate, 96-well Each
12321983 3.5 kDa 0.1 mL Microdialysis Plate, 96-well 1 set
17616415 2 kDa 0.1 mL Microdialysis Plate, 96-well Each
17626415 2 kDa 0.3 mL Microdialysis Plate, 96-well Each
17636415 2 kDa 1 mL Microdialysis Plate, 48-well Each
17324473 3.5 kDa 0.3 mL Microdialysis Plate, 96-well Each
17334473 3.5 kDa 1 mL Microdialysis Plate, 48-well Each
17344473 10 kDa 0.3 mL Microdialysis Plate, 96-well Each
17646415 20 kDa 0.1 mL Microdialysis Plate, 96-well Each
17656415 20 kDa 0.3 mL Microdialysis Plate, 96-well Each
17666415 20 kDa 1 mL Microdialysis Plate, 48-well Each
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 13 options available.
13 options
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Product Code. 17332240 Supplier Thermo Scientific™ Supplier No. A50470

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Description

Simultaneously process 1 to 96 samples with 10 to 100 μL volumes with this convenient rapid dialysis system that is automation compatible and disposable.

Pierce 48-well and 96-well, 2K, 3.5K, 10K, and 20K MWCO microdialysis plates are an automation-compatible microplate with devices for the rapid dialysis of 1 to 96 samples with volumes of 10 to 1000 μL. Using the Pierce Microdialysis Plate, sample dialysis can be efficiently performed in a standard 96 deep-well plate with a minimal amount of buffer while still providing >95% removal of small molecules. The plate is compatible with standard 96-well laboratory equipment and automated liquid-handling systems, making it an ideal option for high-throughput applications.

Each sample processed with the Pierce Microdialysis Plate is dialyzed inside a single, removable dialysis device consisting of two regenerated cellulose membranes ∼2 mm apart. The short diffusion distance and large surface area facilitate rapid dialysis, with the removal of salts and small molecules often complete in 2–4 hours. Sample is recovered using standard laboratory micropipettes. The plate seals are made of high-quality polypropylene and help protect the samples from contamination and evaporation, especially during long-term storage.

Features of Pierce Microdialysis Plate:
• High yield—greater than 90% protein recovery
• Rapid—dialysis completed in 2–4 hours
• Easy to use—sample loading and retrieval can be done with a standard pipette tip
• Flexible—detachable 8-device strips, scalable from 1 to 96 samples
• Automation-compatible—plate format conforms to the SBS Microplate Standard

Each 48-well and 96-well Pierce Microdialysis Plate comes pre-loaded with twelve 8-dialysis device strips. Each dialysis device strip can be removed from the plate and separated into individual microdialysis devices, allowing only the number of needed devices to be used and eliminating waste. The sample chamber inside each microdialysis device is formed by two low-binding regenerated cellulose membranes that are rated to retain proteins and other macromolecules >2,000 Daltons while allowing removal of buffer salts and contaminants

Applications:
• Protein and DNA desalting/buffer exchange
• Removal of dyes after protein labeling
• Enzyme activity/binding assays
• Drug binding studies (also see Rapid Equilbrium Dialysis 'RED' devices)

TRUSTED_SUSTAINABILITY

Specifications

Product Type Microdialysis Plate, 48-well
MWCO 10 kDa
Membrane Cellulose Membrane, Dialysis
Format Microdialysis Plate
Capacity (Metric) 1 mL
Purification Target Buffer Exchange
Sample Volume (Metric) 1 mL
Sufficient For Dialysis of 1 to 48 samples, 150–1000 μL
Quantity 1 Plate Set
Content And Storage Product shipped at ambient temperature. Upon receipt, store at room temperature.

Frequently Asked Questions (FAQs)

Are cartridges from Microdialysis Plates sold separately?

We do not offer the cartridges from Microdialysis plates as stand-alone products. Each individual device from a Microdialysis Plate can be used in a microtube or plate, if the entire strip or plate is not needed.

What is the chemical compatibility of Microdialysis Plates?

Please refer to the Chemical Compatibility table in the respective product manual.
What are 48-well Microdialysis Plates?

The 48-well Microdialysis Plates are automation-compatible 48 deep-well microplates with microdialysis devices for the rapid dialysis of 1 to 48 samples. Each plate includes 8 detachable cartridges where each cartridge is a strip with 6 microdialysis devices.

Why did my sample volume increase during dialysis?

Most likely it‘s because osmotic pressure from dialyzing from high osmolarity to low osmolarity solutions causes a sample volume increase, as each sample component-including water-will move towards equilibrium concentration on both sides of the membrane.
What is the protein recovery after dialysis when using the 96-well Microdialysis plate?

Typical protein recovery is greater than 90% after dialysis.

Do I need to use all 96 wells in the Pierce 96-well microdalysis plate at one time?

The plate comprises detachable strips so that you can pull out the exact number of wells needed for an experiment (scalable from 1-96 samples).
What is the difference between your dialysis devices (plates, device, cassettes, flasks)?

Please view our selection table to choose the right dialysis device for your experiment - https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-purification-isolation/protein-dialysis-desalting-concentration/dialysis-products.html.
What is protein dialysis?

Dialysis is the separation of small and large molecules in a solution by selective diffusion through a semi-permeable membrane. It is generally used for larger sample volumes, and can take hours to overnight for complete dialysis.
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