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  5. Thermo Scientific™ Pierce™ LDH Cytotoxicity Assay Kit

Thermo Scientific™ Pierce™ LDH Cytotoxicity Assay Kit

Product Code. 13454269 Shop All Thermo Scientific Products
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Quantity:
1000 Reaction Kit
200 Reaction Kit
Unit Size:
1000 reactions
200 reactions
Product Code. Quantity unitSize
13454269 200 Reaction Kit 200 reactions
13464269 1000 Reaction Kit 1000 reactions
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Product Code. 13454269

Brand: Thermo Scientific™ 88953

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Description

Quantitatively measure lactose dehydrogenase released into the media from damaged cells as a biomarker for cellular cytotoxicity and cytolysis using a colorimetric assay.

Quantitatively measure lactose dehydrogenase (LDH) released into the media from damaged cells as a biomarker for cellular cytotoxicity and cytolysis using a colorimetric assay with Thermo Scientific™ Pierce LDH Cytotoxicity Assay Kit.

Additional Information and Online Ordering

The Pierce LDH Cytotoxicity Assay Kit measures extracellular LDH in culture media using an enzymatic reaction that results in a red formazan product which can be measured spectrophotometrically. Lactate dehydrogenase (LDH) is a cytosolic enzyme that is is an indicator of cellular toxicity. The assay is ideal for high-throughput screening and provides a safe alternative to radioactive cytotoxicity assays.

Highlights:

  • Convenient – add–mix–read assay format for adherent and suspension cells

  • Colorimetric – quantitatively measures LDH release by formation of colored product

  • Robust – uses stable LDH enzyme activity as a cytotoxic marker

  • Flexible – ideal for high–throughput screening

  • Non–radioactive – safe alternative to 51Cr–release cytotoxicity assays

Includes:

Kit contains substrate mix, assay buffer, 10X lysis buffer, stop solution, and LDH positive control

Requires:

Microplate reader capable of reading absorbance at 490nm and 680nm, flat-bottom clear 96–well plate compatible with spectrophotometer, multichannel pipette

Recommended for:

  • Measure in vitro cytotoxicity mediated by chemicals, immune cells, or siRNA or microRNA
  • Detect cytolysis in bioreactor or 3D tissue engineering applications
TRUSTED_SUSTAINABILITY

Specifications

Description Pierce LDH Cytotoxicity Assay Kit
Quantity 200 Reaction Kit
Kit Contents Substrate Mix, lyophilizate, Stop Solution, 12mL, LDH Positive Control, 6µL, Assay Buffer, 0.6mL, 10X Lysis Buffer, 2.5mL
Sufficient For 200 reactions in 96-well plates or 800 reactions in 384-well plates
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