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Thermo Scientific™ Pierce™ IgM Purification Kit
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Thermo Scientific™ Pierce IgM Purification Kit is an easy-to-use affinity matrix and buffer system to purify IgM from mouse ascites fluid; the method uses immobilized mannan binding protein, which binds to IgM in the kit-supplied buffers.
Pierce MBP Agarose is purified mannan binding protein (also called mannose-binding lectin, MBL) that has been immobilized on 4% beaded agarose. When used with an optimized buffer system based on temperature- and calcium-dependent binding, this resin enables specific affinity purification of mouse IgM from ascites fluid and other concentrated samples. The resin and essential buffers are available individually or bundled together in a complete IgM Purification Kit containing a pre-packed 5mL column of the MBP Agarose.
Highlights:
Immobilized MBP – affinity system based on mannan binding protein (MBP) allows specific purification of mouse IgM from ascites fluid or partial isolation of human IgM from serum
Complete kit – IgM Purification Kit includes a pre-packed MBP column and ready-to-use buffers for convenient purification of at least 1.5mg IgM from 0.5mL ascites samples per column-use
Mild conditions – nondenaturing, near-neutral buffer conditions ensure recovery of functional antibodies
Reusable – stable ligand and resins can be regenerated and reused at least 10 times without significant decline in binding capacity
Includes:
Kit contains a 5mL MBP Agarose Column, column preparation buffer, IgM binding and elution buffers, and a column extender
Specifications
Specifications
| Product Type | IgM Purification Kit |
| Product Line | Pierce™ |
| Column Type | Agarose Resin, Affinity |
| Format | Kit |
| Stationary Phase | Lectin |
| Purification Target | IgM Antibody |
| Description | Pierce IgM Purification Kit |
| Content And Storage | Sufficient For: 10 IgM purifications, each from 0.5 mL mouse ascites samples • MBP Agarose Column, 5 mL, 1 column • IgM Binding Buffer, 800 mL • IgM Elution Buffer, 500 mL • MBP Column Preparation Buffer, 50 mL • Column Extender Store at 4°C |
| Quantity | 1 Column |
For Research Use Only. Not for use in diagnostic procedures.
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