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Thermo Scientific™ Pierce™ High Capacity Protein A MagBeads, alkali stable
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Pierce High-Capacity Protein A MagBeads, Alkali Stable, are high-performance magnetic beads for purification of antibodies from cell culture supernatant, ascites, serum, and crude extracts.
Features of the High-Capacity Protein A MagBeads include:
- High binding capacity—≥ 40 mg HIgG/mL settled beads
- Alkaline stable—engineered to withstand standard CIP procedures with 0.1 M NaOH, enabling efficient cleaning of the beads for reuse
- Animal component-free raw materials
- Versatile—beads are compatible with manual and automated workflows (e.g., KingFisher instruments)
- Stable—high-affinity Protein A is securely immobilized to magnetic agarose to help prevent leaching of ligand, target antibody, or base support
The protein A ligand has been genetically modified for stability in the presence of NaOH, so the beads can be regenerated at least 10 times without sacrificing binding capacity. The ligand is produced in yeast and is immobilized on magnetic agarose with an average particle size of ∼30 μm.
Pierce High-Capacity Protein A MagBeads, Alkali-Stable, are suitable for purifying mAbs and a subpopulation of scFv's and Fab fragments containing a VH3 domain. The beads are compatible with automation, allowing for higher throughput purifications and screening of purification conditions.
Specifications
Specifications
| Bed Volume | 0.25 mL |
| Binding Capacity | ≥40 mg HIgG/mL settled beads |
| Capacity (Metric) | 1 mL |
| Concentration | Slurry: 25% |
| Description | High Capacity Magnetic Agarose Beads |
| Content And Storage | Provided in the form of a 25% slurry. Upon receipt store at 2–8°C. |
| Particle Size | 25 to 30 μm |
| Quantity | 1 mL |
| For Use With (Application) | Antibody Purification |
| Ligand Type | Alkaline Stable Protein A |
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For Research Use Only. Not for use in diagnostic procedures.
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