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  6. Thermo Scientific™ Pierce™ Glutathione Superflow Agarose

Thermo Scientific™ Pierce™ Glutathione Superflow Agarose

Product Code. 13454199 Shop All Thermo Scientific Products
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Product Code. Quantity unitSize
13454199 250 mL 250mL
13434199 50 mL 50mL
13444199 1 L 1 L
13424199 10 mL 10mL
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Product Code. 13454199

Brand: Thermo Scientific™ 25238

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Description

Perform high-purity, large-scale purification of GST-tagged proteins with glutathione that has been immobilized to a crosslinked 6% beaded agarose.

Perform high-purity, large-scale purification of GST-tagged proteins with Thermo Scientific™ Pierce Glutathione Superflow Agarose, which consists of glutathione that has been immobilized to a crosslinked 6% beaded agarose.

Additional Information and Online Ordering

Pierce Glutathione Superflow Agarose is a highly crosslinked, durable resin that does not compress at flow rates required for medium- to large-scale FPLC purifications. The resin holds up well to a variety of chemical and pH values, and it is compatible with common clean-in-place procedures. The specific binding of glutathione to the GST-tag results in high purity and high yield in a single step purification process.

Highlights:

  • High dynamic binding capacity – binds 10mg of pure GST per mL of resin at a linear flow rate of 150cm/hour; can bind greater than 20mg/mL at 30cm/hour flow rate

  • High purity – provides greater than 90% purity when used to purify overexpressed GST or GST-fusion proteins from lysates

  • Robust – highly crosslinked 6% agarose Superflow beaded support tolerates linear flow rates up to 1200cm/hour

  • Compatible – maintains function after exposure to a wide variety of chemicals and pH values

  • Cost effective – resin is stable through multiple cycles of cleaning and reuse

Recommended for:

Medium- to large-scale FPLC purification of GST-tagged proteins

TRUSTED_SUSTAINABILITY

Specifications

Capacity (Metric) 10 mg Pure GST Binding per mL Resin at 150 cm/hour Flow Rate, 20 mg per mL at 30 cm/hour Flow Rate
Content And Storage Upon receipt store at 4°C.
Product Line Pierce
Quantity 250 mL
Target GST-Tagged Protein
Type Agarose
Stationary Phase Glutathione
Format Bottle
Column Type Affinity, Agarose Resin

For Research Use Only. Not for use in diagnostic procedures.

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