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Thermo Scientific™ Fluoraldehyde™ o-Phthaldialdehyde Crystals (OPA)
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Thermo Scientific™ Pierce Fluoraldehyde (OPA) is o-phthalaldehyde, a highly sensitive fluorescent pre- or post-column derivatization reagent for peptide or amino acid detection and quantitation in HPLC.
Fluoraldehyde Crystals are a specially purified, fluorogenic-grade preparation of o-phthalaldehyde (OPA). This amine-reactive compound is primarily used for high-sensitivity, fluorescent detection of primary amines, including amino acids, peptides, proteins and polyamines in pre- or post-column chromatographic effluents.
Highlights:
For pre- or post-column amino acid derivatization for fluorescent detection and quantitation
Reacts with all primary amine-containing analytes to yield fluorescent derivatives (ex/em = 340nm/455nm)
Provides an accurate measure of both composition and absolute protein-peptide content
Ideal for work with recombinant proteins and synthetic peptides
Can be used for fluorescent protein or peptide assay
Can be prepared as a stable aqueous solution in borate (see instructions)
Reagent Properties:
Can be prepared as a stable aqueous solution in borate (see instructions)
Alternative names: Fluoraldehyde Crystals, OPA
Chemical name: ortho-Phthalaldehyde
Reactive toward: Primary amines (–NH2)
Chemical formula: C8H6O2
CAS number: 643-79-8
Molecular weight: 134.12
Form: Pale yellow, free-flowing crystals
Specifications
Specifications
| Product Type | o-Phthaldialdehyde |
| Chemical Reactivity | Amine |
| Product Line | Fluoraldehyde™ |
| Content And Storage | Store at room temperature. |
| Label or Dye | OPA (o-phthaldialdehyde) |
| Detection Method | Fluorescence |
| Quantity | 5 g |
| Form | Solid |
Safety and Handling
- o-Phthalaldehyde
- Danger
- Acute toxicity Category 3
- Serious eye damage/eye irritation Category 1
- Skin corrosion/irritation Category 1 B
- Skin sensitiser Category 1
- H301-Toxic if swallowed.
- H314-Causes severe skin burns and eye damage.
- H317-May cause an allergic skin reaction.
- H318-Causes serious eye damage.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P264-Wash thoroughly after handling.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P405-Store locked up.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contains : phthalaldehyde
For Research Use Only. Not for use in diagnostic procedures.
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