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Invitrogen™ PDGF-AB Human ELISA Kit
Sandwich ELISA Kit
Brand: Invitrogen™ EHPDGFABX5
Additional Details : Weight : 0.33820kg
Description
The Human Platelet Derived Growth Factor-AB (Hu PDGFAB) ELISA quantitates Hu PDGFAB in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu PDGFAB. Principle of the method The Human PDGFAB solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Platelet-Derived Growth Factor (PDGF) is a mitogenic peptide growth hormone carried in the alpha-granules of platelets and is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication. The synthesis of PDGF can be induced by IL-1, IL-6, TNF-alpha, TGF-beta and EGF.Specifications
| P01127,P04085 | |
| 10 pg/mL | |
| Solid-phase sandwich ELISA | |
| ELISA Kit | |
| Plasma, Supernatant, Serum | |
| 5 x 96 assays | |
| ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis | |
| 5154, 5155 | |
| <12% | |
| Biotinylated Detection Antibody, Chromogen, SAV-HRP, Standard, Pre-coated 96 well plates, Assay Diluent Concentrate, Wash Buffer, Stop Solution, Adhesive Plate Covers | |
| 5 x 96 Tests | |
| Neurobiology | |
| 2°C to 8°C | |
| Human | |
| 4 hr. 45 min. |
| 10.24 pg/mL to 2500 pg/mL | |
| 10 pg/mL to 2500 pg/mL | |
| Biotin | |
| Growth Factors & Receptors | |
| This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF-BB, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF- alpha, TNF-beta, TPO, VEGF | |
| Human | |
| Colorimetric Microplate Reader | |
| IBGC5,PDGF-2,PDGF2,SIS,SSV,c-sis,PDGF-A,PDGF1 | |
| <10% | |
| HRP | |
| RUO | |
| Plasma, 50 μ;L; Serum, 50 μ;L; Supernatant, 100 μ;L | |
| LL22NC03-10C3.2, IBGC5, PDGF-2, PDGF2, SIS, SSV, c-sis | |
| 1 hr. 20 min. |