Learn More
Invitrogen™ PARN Human ELISA Kit
Sandwich ELISA Kit
Brand: Invitrogen™ EHPARNX5
Additional Details : Weight : 0.33820kg
Description
The Human PARN (DAN) ELISA quantitates Hu PARN in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu PARN. Principle of the method The Human PARN solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
The protein encoded by this gene is a 3′-exoribonuclease, with similarity to the RNase D family of 3′-exonucleases. It prefers poly(A) as the substrate, hence, efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.Specifications
O95453 | |
35 pg/mL | |
Solid-phase sandwich ELISA | |
ELISA Kit | |
Plasma, Serum, Supernatant | |
5 x 96 assays | |
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis | |
5073 | |
<12% | |
SAV-HRP, Stop Solution, Wash Buffer, Chromogen, Pre-coated 96 well plates, Assay Diluent Concentrate, Adhesive Plate Covers, Biotinylated Detection Antibody, Standard | |
5 x 96 Tests | |
Cell Cycle & Proliferation | |
2°C to 8°C | |
Human | |
4 hr. 45 min. |
27.43 pg/mL to 20,000 pg/mL | |
35 pg/mL to 20,000 pg/mL | |
Biotin | |
Cell Structure & Organelle Proteins | |
This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL- 11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF- 7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. | |
Human | |
Colorimetric Microplate Reader | |
DAN,DKCB6,PFBMFT4 | |
<10% | |
HRP | |
RUO | |
Plasma, 50 μ;L; Serum, 50 μ;L; Supernatant, 100 μ;L | |
DAN | |
1 hr. 20 min. |