missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Biochemical Reagents
  4. DNA Reagents
  5. Plasmid DNA
  6. Macherey-Nagel™ NucleoSpin™ 96 Plasmid Transfection-grade Core Kit, 96-well kit

Macherey-Nagel™ NucleoSpin™ 96 Plasmid Transfection-grade Core Kit, 96-well kit

Product Code. 15533636
Change view
Click to view available options
No. of Samples:
24 x 96
4 x 96
Product Type:
Core Kit
Full Kit
Unit Size:
2304 reactions
384 reactions
Product Code. No. of Samples Product Type unitSize
15533636 4 x 96 Full Kit 384 reactions
15553636 4 x 96 Core Kit 384 reactions
15543636 24 x 96 Core Kit 2304 reactions
3 options
This item is not returnable. View return policy

Product Code. 15533636

Brand: Macherey-Nagel™ 740491.4

Please to purchase this item. Need a web account? Register with us today!

This item is not returnable. View return policy

Description

A fast way to purify transfection-grade plasmid DNA using mini-format technology to reduce endotoxins from bacterial lysates. Full and Core kits utilize a 96-well plate format for an efficient high-throughput process.

Endotoxins are co-purified during typical plasmid preparations from bacterial lysates. Since they interfere with eukaryotic cell survival, endotoxin reduction is essential prior to cell transfection. NucleoSpin™ Plasmid Transfection-grade kits are designed to reduce endotoxins from bacterial lysates.

  • Purifies high-copy plasmids for transfection
  • Novel technology to diminish endotoxin content (< 50 EU/μg DNA) for successful transfection
  • Rapid purification from E. coli via automatic, manual, vacuum or centrifugation processing
  • Ultra-fast procedure simplifies the work-flow
  • High-throughput — 96-well plates allow for easy automation and supports plasmid screening
  • Downstream applications: transfection of common cell lines (lipofection, electroporation)
  • Also suitable for standard molecular biology application, such as cloning and sequencing

Specifications

Bacterial Endotoxins <50 EU/ng DNA
Content And Storage Room temperature (18 - 25 °C)
Format 96-well Plate
Isolation Technology Silica Membrane and Endotoxin-reduction Technology
Sample Type Bacterial Culture (E. coli, High-copy Plasmids)
Efficiency Binding capacity: 20 μg
Elution Volume Elution Volume: 100 - 200 μL
Final Product Type Transfection-grade plasmid DNA
For Use With (Equipment) Centrifuge, Vacuum manifold (e.g. NucleoVac 96)
For Use With (Application) Rapid purification of plasmids for transfection
Includes Filter Plates, Binding Plates, MN Wash Plates, Culture Plates, Elution Plates, Buffers, RNase A
No. of Samples 4 x 96
Product Type Full Kit
Purity A260/A280: 1.80 - 1.85
Sample Volume Up to 5 mL
Size < 15 kbp
Test Time 45 min/plate
Yield 5 - 20 μg
Show More Show Less

Safety and Handling

compliance-icons compliance-icons compliance-icons
Product Identifier
  • NucleoSpin 96 Plasmid T.-grade (4x96)
 Signal Word
  • Danger
 Hazard Category
  • Acute toxicity Category 4
  • Serious eye damage/eye irritation Category 2
  • Flammable liquid Category 3
  • Respiratory sensitiser Category 1
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity Category 3
 Hazard Statement
  • H226-Flammable liquid and vapour.
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
  • H336-May cause drowsiness or dizziness.
 Precautionary Statement
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P264-Wash thoroughly after handling.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
  • P330-Rinse mouth.
  • P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
 Supplemental information
  • MIXTURE LIST-Contains : sodium hydroxide, dodecyl sulphate, guanidine hydrochloride,triethanolamine, 2-propanol, RNAse
Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.