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Macherey-Nagel™ NucleoSpin™ 96 Plasmid Transfection-grade Core Kit, 96-well kit
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No. of Samples:
24 x 96
4 x 96
Product Type:
Core Kit
Full Kit
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2304 reactions
384 reactions
Beschreibung
Endotoxins are co-purified during typical plasmid preparations from bacterial lysates. Since they interfere with eukaryotic cell survival, endotoxin reduction is essential prior to cell transfection. NucleoSpin™ Plasmid Transfection-grade kits are designed to reduce endotoxins from bacterial lysates.
- Purifies high-copy plasmids for transfection
- Novel technology to diminish endotoxin content (< 50 EU/μg DNA) for successful transfection
- Rapid purification from E. coli via automatic, manual, vacuum or centrifugation processing
- Ultra-fast procedure simplifies the work-flow
- High-throughput — 96-well plates allow for easy automation and supports plasmid screening
- Downstream applications: transfection of common cell lines (lipofection, electroporation)
- Also suitable for standard molecular biology application, such as cloning and sequencing
Spezifikation
Spezifikation
| Bacterial Endotoxins | <50 EU/ng DNA |
| Content And Storage | Room temperature (18 - 25 °C) |
| Format | 96-well Plate |
| Isolation Technology | Silica Membrane and Endotoxin-reduction Technology |
| Sample Type | Bacterial Culture (E. coli, High-copy Plasmids) |
| Efficiency | Binding capacity: 20 μg |
| Elution Volume | Elution Volume: 100 - 200 μL |
| Final Product Type | Transfection-grade plasmid DNA |
| For Use With (Equipment) | Centrifuge, Vacuum manifold (e.g. NucleoVac 96) |
| For Use With (Application) | Rapid purification of plasmids for transfection |
| Mehr anzeigen |
Sicherheit und Handhabung
Produktidentifikator
- NucleoSpin 96 Plasmid T.-grade (4x96)
- Danger
- Acute toxicity Category 4
- Serious eye damage/eye irritation Category 2
- Flammable liquid Category 3
- Respiratory sensitiser Category 1
- Skin corrosion/irritation Category 2
- Specific target organ toxicity Category 3
- H226-Flammable liquid and vapour.
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
- H336-May cause drowsiness or dizziness.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P264-Wash thoroughly after handling.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
- P330-Rinse mouth.
- P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
- MIXTURE LIST-Contains : sodium hydroxide, dodecyl sulphate, guanidine hydrochloride,triethanolamine, 2-propanol, RNAse
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