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Invitrogen™ Human IP-10 (CXCL10) ELISA Kit

Product Code. 10640734
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Quantity:
96 Tests
Unit Size:
96 tests
Product Code. Quantity unitSize
10640734 96 Tests 96 tests
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Product Code. 10640734

Brand: Invitrogen™ KAC2361

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Description

ELISA

The Human Inflammatory Protein-10 (Hu IP-10/CXCL10) ELISA quantitates Hu IP-10/CXCL10 in human serum, plasma, saliva, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IP-10. Principle of the method The Human IP-10/CSCL10solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IP-10 (interferon-gamma induced protein 10 kD; in mouse, also called cytokine responsive gene 2, Crg-2, or mob-1) is a highly inducible, primary response gene that belongs to the CXC chemokine superfamily. It was first cloned in 1985. The biological functions of IP-10 are still unclear. Like Mig, IP-10 has no activity on neutrophils. Its functions include stimulation of monocytes, natural killer and T-cell migration, regulation of T-cell and bone marrow progenitor maturation, modulation of adhesion molecule expression as well as inhibition of angiogenesis. Mig and IP-10 share the same receptor, CXCR3.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P02778
Assay Range 7.8 to 500 pg/mL
Assay Sensitivity <2 pg/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Saliva, Serum, Supernatant
Target Species Human
For Use With (Application) Colorimetric Detection
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 3627
Gene Symbol CXCL10
Incubation Time 4 hr.
Interassay CV 8.4%
Intraassay CV 4.3
Kit Contents Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Marker Gene IP-10
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Saliva 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Target CXCL10 (IP-10)
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs
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