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Invitrogen™ Estradiol Human ELISA Kit

Product Code. 10630354
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Quantity:
96 Tests
Unit Size:
96 tests
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Product Code. 10630354

Brand: Invitrogen™ KAQ0621

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Description

Includes

Pre-coated plate, standard, controls, anti-estradiol, estradiol-HRP, diluents, wash buffer, chromogen, and stop solution.

Sandwich ELISA Kit

The Human Estradiol ELISA quantitates Hu 17-beta Estradiol in human serum and plasma. The assay will exclusively recognize both natural and recombinant Hu Estradiol. Principle of the method The Human Estradiol solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Estradiol (17beta-estradiol) is a sex hormone. Estradiol is the predominant sex hormone present in females. It is also present in males, being produced as an active metabolic product of testosterone. It represents the major estrogen in humans. Estradiol has not only a critical impact on reproductive and sexual functioning, but also affects other organs including the bones.

Order Info

Shipping Condition: Dry Ice

TRUSTED_SUSTAINABILITY

Specifications

Assay Range 13-935 pg/mL
Assay Sensitivity 5 pg/mL
Conjugate HRP
Product Type ELISA Kit
Sample Type Plasma, Serum
Target Species Human
For Use With (Application) Colorimetric Detection
For Use With (Equipment) Colorimetric Microplate Reader
Incubation Time 2.5 hr.
Interassay CV 6.1%
Intraassay CV 4.3%
Kit Contents Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Incubation Buffer, HRP conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Controls
Label or Dye HRP
Marker Gene Estradiol
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Plasma, 50 μL; Serum, 50 μL
Storage Requirements 2°C to 8°C
Target Human
Test Time 1 hr. 20 min.
Total Assay Time 2 hr. 30 min.
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For Research Use Only. Not for use in diagnostic procedures.

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