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Invitrogen™ Mouse IL-17E/IL-25 Uncoated ELISA Kit with Plates

Product Code. 15520987
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Quantity:
2 x 96 Tests
Unit Size:
Pack of 2
Product Code. Quantity unitSize
15520987 2 x 96 Tests Pack of 2
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Product Code. 15520987

Brand: Invitrogen™ 88700222

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Description

ELISA

The Mouse Interleukin 17E/Interleukin 25 (IL-17E) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse IL-17E. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Interleukin 25 (IL-25) is a member of the IL-17 family and is identified as a proinflammatory cytokine which is produced by Th17 effector cells thus enabling the Th17 subset to initiate or maintain the autoimmune process in diseases such as Rheumatoid Arthritis. IL-25 also induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. IL-25 itself is activated through IL-23, IL-5, and TGF-beta.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number Q8VHH8
Assay Range 32-2000 pg/mL
Assay Sensitivity 32 pg/mL
Content And Storage Capture Antibody: Pre-titrated, purified antibody
Detection Antibody: Pre-titrated, biotin-conjugated antibody
Standard: Recombinant cytokine for generating standard curve and calibrating samples
Coating Buffer: 10X PBS ELISA Coating Buffer
Assay Diluent: 5X Concentrated
Sample Diluent B: 12 mL of a 1X solution per plate
Detection Enzyme: Pre-titrated Avidin-HRP
Substrate Solution: Tetramethylbenzidine (TMB) Solution
Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards.
96-well Plates: Corning Costar flat-bottom plates (included with Cat. Nos. ending in -22 and -86)
Detectable Analytes IL-17 E (IL-25)
Assay 16-2,000 pg/ml
Conjugate HRP
Immunoassay Kit Format Uncoated ELISA Kit with Plates
Product Type ELISA
Sample Type Serum, Supernatant
Target Species Mouse
For Use With (Application) ELISA
For Use With (Equipment) Absorbance Microplate Reader
Gene Alias D17Wsu104e; il 17e; Il17e; IL-17E; Il25; IL-25; ILN; Interleukin; interleukin 17E; interleukin 25; interleukin-17E; Interleukin-25; Ly6elg; Mydgf; myeloid derived growth factor; myeloid-derived growth factor; RGD1561632; UNQ3120/PRO10272; UPF0556 protein C19orf10 homologD17Wsu104e; il 17e; Il17e; IL-17E; Il25; IL-25; ILN; Interleukin; interleukin 17E; interleukin 25; interleukin-17E; Interleukin-25; Ly6elg; Mydgf; myeloid derived growth factor; myeloid-derived growth factor; RGD1561632; UNQ3120/PRO10272; UPF0556 protein C19orf10 homolog; BSF-2; BSF2; CDF; HGF; HSF; IFN-beta-2; IFNB2; IL-6
Gene ID (Entrez) 140806
Gene Symbol IL25
Host Species Mouse
Kit Contents Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, biotin-conjugated antibody, Standard: Recombinant cytokine for generating standard curve and calibrating samples, Coating Buffer: 10X PBS ELISA Coating Buffer, Assay Diluent: 5X Concentrated, Sample Diluent B: 12 mL of a 1X solution per plate, Detection Enzyme: Pre-titrated Streptavidin-HRP, Substrate Solution: Tetramethylbenzidine (TMB) Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22 and -86), Purified antibody, Detection Antibody: Pre-titrated, Biotin-conjugated antibody, Standard: Recombinant cytokine for generating standard curve and calibrating samples, Coating Buffer: 10X PBS ELISA Coating Buffer, Assay Diluent: 5X Concentrated, Sample Diluent B: 12 mL of a 1X solution per plate, Detection Enzyme: Pre-titrated Streptavidin-HRP, Substrate Solution: Tetramethylbenzidine (TMB) Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22 and -86)
Label or Dye Biotin
Quantity 2 x 96 Tests
Regulatory Status RUO
Sample Volume Serum 50 μL, Supernatant 100 μL
Storage Requirements 2-8°C
Target IL-25
Test Time 1 hr 45 min
Total Assay Time 24 hr 30 min
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