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  5. Invitrogen™ Human MIP-3 alpha/CCL20 Cell Lysate ELISA Kit

Invitrogen™ Human MIP-3 alpha/CCL20 Cell Lysate ELISA Kit

Product Code. 15387118
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Quantity:
10 x 96 Tests
5 x 96 Tests
96 Tests
Förpackningsstorlek
1 set
Each
Pack of 10
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Produktkod. 15387118

missing translation for 'mfr': Invitrogen™ EHCCL20CL

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Beskrivning

ELISA

The Human MIP-3 alpha/CCL20 ELISA quantitates Hu MIP-3α in human cell or tissue lysates. The assay will exclusively recognize both natural and recombinant Hu MIP-3α. Principle of the method The Human MIP-3α solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Macrophage Inflammatory Protein-3 alpha (MIP-3alpha), also called CCL20, is expressed in the liver, lungs, lymph nodes and peripheral blood lymphocytes. MIP-3alpha is strongly up regulated by inflammatory signals, and down regulated by the anti-inflammatory cytokine IL-10. MIP3alpha signals through the G protein-coupled receptor, CCR6, and acts as a chemoattractant to lymphocytes and dendritic cells.
TRUSTED_SUSTAINABILITY

Specifikationer

Accession Number P78556
Assay Range 0.8 to 600 pg/mL
Assay Sensitivity 1.5 pg/mL
Calibration Range 1.5 pg/mL to 600 pg/mL
Assay Solid-phase sandwich ELISA
Conjugate HRP
Product Type ELISA
Protein Family Chemokine & Receptors
Sample Type Cell Lysates
Specificity The antibody pair provided in this kit recognizes human MIP-3 alpha.
Sufficient For 96 Assays
Target Species Human
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 6364
Gene Symbol CKb4,Exodus,LARC,MIP-3-alpha,MIP-3 A,MIP3A,SCYA20,ST38
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Cell Lysis Buffer, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Research Discipline Inflammation
Sample Volume Cell Lysate 20 μL
Storage Requirements 2°C to 8°C
Synonym CKb4, Exodus, LARC, MIP-3-alpha, MIP-3 A, MIP3A, SCYA20, ST38
Target CCL20
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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