missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Lysates
  5. Cell Line Lysates
  6. 16069545

Jurkat, clone E6-1 (human acute T cell leukemia) Whole cell lysate, Non-denatured; Abnova

Product Code. 16069545
Click to view available options
Quantity:
200 μg
Unit Size:
200µg
This item is not returnable. View return policy

Product Code. 16069545

Brand: Abnova L017V2.200ug

Please to purchase this item. Need a web account? Register with us today!

This item is not returnable. View return policy

Description

Non-denatured whole cell lysate of Jurkat, clone E6-1 (human acute T cell leukemia)

  • Tissue: T Lymphocyte
  • Host: Human
  • Lysis Buffer: Modified RIPA lysis buffer: 50mM Tris-HCl pH 7.4, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
  • Storage buffer: In modified RIPA lysis buffer

Western Blotting, Immunoprecipitation

Specifications

For Use With (Application) Immunoprecipitation, Western Blot
Tissue T Lymphocyte
Description Whole cell lysate (non-denatured)
Lysis Buffer Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Preparation Method Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Quality Control Testing 12.5% SDS-PAGE Stained with Coomassie Blue.
Quantity 200 μg
Storage Buffer In modified RIPA Lysis Buffer.
Host Species Human
Content And Storage Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Ihr Feedback wurde übermittelt