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Invitrogen™ β-Gal Assay Kit

Product Code. 10093072
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Quantity:
80 mL
Unit Size:
5mL
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10093072 80 mL 5mL
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Product Code. 10093072 Supplier Invitrogen™ Supplier No. K145501

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Description

Includes

10X PBS (phosphate buffered saline), lysis buffer, cleavage buffer, β-mercaptoethanol, ONPG, stop buffer.

Provides reagents required to quickly measure levels of active β-galactosidase expressed in cells transfected with plasmids expressing lacZgene

  • lacZis bacterial gene often used as reporter construct in eukaryotic transfection experiments
  • Gene product of lacZ, β-galactosidase, is resistant to proteolysis in cellular lysates, and its activity is easily assayed
  • β-galactosidase catalyzes hydrolysis of β-galactosidase such as ortho-nitrophenyl-D-galactopyranoside (ONPG)
  • Hydrolysis of ONPG to ONP anion produces bright yellow color with peak absorbance at 420nm that can be quantified using spectrophotometer
  • 10.7 gene expression
  • Colorimetric detection

Beta-Galactosidase Assays, Enzyme and Protein Activity Assays, Proteins, Expression, Isolation and Analysis, Reporter Assays

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage The β-Gal Assay Kit contains 10X PBS (phosphate buffered saline), lysis buffer, cleavage buffer, β-mercaptoethanol, ONPG, stop buffer. Store ONPG at -20°C. All other components are stored at either room temperature or +4°C. All reagents are guaranteed stable for 6 months when properly stored.
Assay β-Gal (lacZ) Assay
Detection Method Colorimetric
Product Type Beta-Gal Assay Kit
Substrate ONPG
Quantity 80 mL
Target Beta-Galactosidase

Frequently Asked Questions (FAQs)

Are detergents compatible with the β-Gal (ONPG) assay kit (K1455-01)?

Neither NP40 nor Triton X-100 (1%) should interfere with the reaction, although there is a chance that a precipitate may form when the detergent is added to the b-Gal assay reagent.
How do I resuspend IPTG and X-Gal?

IPTG can be reconstituted in water. Make a stock of 100 mM in water and store working aliquots at -20°C. X-gal can be reconstituted in DMSO, or in a 50:50 mix of DMSO and water. To do the latter, you must dissolve in DMSO first, and then add water to bring up to final volume. It is not necessary to filter sterilize these solutions.

The X-gal solution should be protected from light. To make plates, add 50 ug/ml X-gal and 1 mM (0.24 mg/mL) IPTG to LB/agar that has been cooled down to 50°C. To spread on top of plates, use 50 µl 2% stock of X-gal and 30 µl 100 mM stock of IPTG. 

For Research Use Only. Not for use in diagnostic procedures.

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