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  5. Invitrogen™ Human IgA ELISA Kit

Invitrogen™ Human IgA ELISA Kit

Product Code. 15561637
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Quantity:
10 x 96 Tests
96 Tests
Unit Size:
96 tests
Pack of 10
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15561637 96 Tests 96 tests
15581637 10 x 96 Tests Pack of 10
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Product Code. 15561637 Supplier Invitrogen™ Supplier No. BMS2096

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Description

ELISA

The Human ImmunoGlobulin A (Hu IgA) ELISA quantitates Hu IgA in human serum or plasma. The assay will exclusively recognize both natural and recombinant Hu IgA. Principle of the method The Human IgA solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Human IgA, also known as human immunoglobulin A, is an antibody that is produced by the immune system in response to antigens. It is the most abundant immunoglobulin in mucosal secretions, such as saliva, tears, and breast milk, and plays a key role in defense against pathogens at mucosal surfaces. IgA works by binding to specific antigens and preventing them from entering the body's tissues. It can also activate complement and promote phagocytosis by immune cells. Human IgA can be used in research to study the immune response to pathogens and to develop diagnostic tests and therapeutic interventions for infectious diseases.
TRUSTED_SUSTAINABILITY

Specifications

Assay Range 1.6 to 100 ng/mL
Assay Sensitivity 0.03 ng/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum
Target Species Human
For Use With (Application) ELISA
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 3493, 3494
Interassay CV 4.8%
Intraassay CV 3.9
Kit Contents Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Plasma 10 μL, Serum 10 μL
Storage Requirements 2°C to 8°C
Synonym Immunoglobulin A
Target Human IgA
Test Time 1 hrs 20 mins
Total Assay Time 1 hrs 30 mins
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