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Proteintech™ Human TIMP2 ELISA Kit

Product Code. 16891566 Shop All Proteintech Products
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Target Species:
Human
Unit Size:
96 tests
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Product Code. 16891566

Brand: Proteintech™ KE0016296T

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This item is not returnable. View return policy

Description

KE00162 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The TIMP2 ELISA kit is to be used to detect and quantify protein levels of endogenous TIMP2.

  • The assay recognizes human TIMP2. An antibody specific for TIMP2 has been pre-coated onto the microwells
  • The TIMP2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human TIMP2 is added to detect the captured human TIMP2 protein.
  • For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.
  • TIMP2, a 21-kDa protein, is a member of the family of matrix metalloproteinase inhibitors, which contains four members (TIMP1, TIMP2, TIMP3, and TIMP4).
  • Tissue inhibitors of metalloproteinases (TIMPs) are multifaceted molecules that exhibit properties beyond their classical proteinase inhibitory function.
  • TIMP-2 is the only TIMP functioning as both an MMP inhibitor and an activator, because it can interact with membrane type 1 MMP and pro-MMP2 to facilitate the activation of pro-MMP2 to its active form.
  • TIMP2 can regulate many physiological progression such as tumor growth, metastasis, and angiogenesis through matrix metalloproteinases (MMPs).
  • TIMP2 knockout resulted in constitutive MMP9 activation abundantly and adverse MI remodeling in vivo.

Specifications

Accession Number BC071586
Assay Range 31.25 to 2000 pg/mL
Assay Sensitivity 0.3 pg/mL
Certifications/Compliance ISO 13485
Detection Method Sandwich ELISA, Double Antibody
Immunoassay Kit Format Coated ELISA Kit
Sample Type Plasma, Serum
Substrate DAB
Target Species Human
Gene ID (Entrez) 7077
Host Species Human
Immunoassay Kit Grade Solid
Interassay CV <10%
Intraassay CV <10%
Shipping Condition Blue ice
Storage Requirements -20°C to +4°C
Total Assay Time 4.5 hr.
UniProt ID P16035
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Safety and Handling

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Product Identifier
  • 96TEST Human TIMP2 ELISA Kit
 Signal Word
  • Warning
 Hazard Category
  • Serious eye damage/eye irritation Category 1
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity Category 3
 Hazard Statement
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H318-Causes serious eye damage.
  • H319-Causes serious eye irritation.
  • H332-Harmful if inhaled.
  • H335-May cause respiratory irritation.
 Precautionary Statement
  • P201-Obtain special instructions before use.
  • P202-Do not handle until all safety precautions have been read and understood.
  • P234-Keep only in original container.
  • P264-Wash skin thoroughly after handling.
  • P270-Do not eat, drink or smoke when using this product.
  • P273-Avoid release to the environment.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P308+P313-IF exposed or concerned: Get medical advice/attention.
  • P330-Rinse mouth.
  • P362-Take off contaminated clothing.
  • P405-Store locked up.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : Sulphuric Acid, Tetramethylbenzidine
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