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Proteintech™ Human/Rat BDNF ELISA Kit
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Target Species:
Human, Rat
Unit Size:
96 tests
Description
- The assay recognizes human BDNF. An antibody specific for BDNF has been pre-coated onto the microwells. The BDNF protein in samples is captured by the coated antibody after incubation.
- Following extensive washing, another antibody of biotinylated specific for human BDNF is added to detect the captured human BDNF protein.
- For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
- The brain-derived neurotrophic factor (BDNF), as a member of the neurotrophin family of growth factors, is broadly expressed in the central nervous system.
- BDNF is involved in cerebral morphogenesis, synaptic plasticity and learning, mental health, degenerative central nervous system (CNS) diseases, and energy metabolism.
- BDNF has recently been identified as one of the four major pharmaceutical targets in neuropsychiatric diseases, including depression and bipolar disorder.
- In addition, BDNF is increasingly considered as a potential therapeutic agent for neurodevelopmental disorders such as Rett syndrome.
Specifications
Specifications
| Accession Number | NP_001137277 |
| Assay Range | 12.5 to 800 pg/mL |
| Assay Sensitivity | 0.1 pg/mL |
| Certifications/Compliance | ISO 13485 |
| Detection Method | Sandwich ELISA, Double Antibody |
| Immunoassay Kit Format | Coated ELISA Kit |
| Sample Type | Plasma, Serum |
| Substrate | DAB |
| Target Species | Human, Rat |
| Gene ID (Entrez) | 627 |
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Safety and Handling
Product Identifier
- 96TEST Human/Rat BDNF ELISA Kit
- Warning
- Serious eye damage/eye irritation Category 1
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- Specific target organ toxicity Category 3
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H318-Causes serious eye damage.
- H319-Causes serious eye irritation.
- H332-Harmful if inhaled.
- H335-May cause respiratory irritation.
- P201-Obtain special instructions before use.
- P202-Do not handle until all safety precautions have been read and understood.
- P234-Keep only in original container.
- P264-Wash skin thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P273-Avoid release to the environment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P308+P313-IF exposed or concerned: Get medical advice/attention.
- P330-Rinse mouth.
- P362-Take off contaminated clothing.
- P405-Store locked up.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contains : Sulphuric Acid, Tetramethylbenzidine
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