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  5. Invitrogen™ Human PLA2G2A ELISA Kit

Invitrogen™ Human PLA2G2A ELISA Kit

Product Code. 17104673
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Quantity:
5 x 96 Tests
96 Tests
Unit Size:
Each
Pack of 96
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Product Code. 17104673

Brand: Invitrogen™ EH369RB

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Description

ELISA

Human PLA2G2A quantitates human PLA2G2A in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human PLA2G2A.

The protein encoded by this gene is a member of the phospholipase A2 family (PLA2). PLA2s constitute a diverse family of enzymes with respect to sequence, function, localization, and divalent cation requirements. This gene product belongs to group II, which contains secreted form of PLA2, an extracellular enzyme that has a low molecular mass and requires calcium ions for catalysis. It catalyzes the hydrolysis of the sn-2 fatty acid acyl ester bond of phosphoglycerides, releasing free fatty acids and lysophospholipids, and thought to participate in the regulation of the phospholipid metabolism in biomembranes. Several alternatively spliced transcript variants with different 5' UTRs have been found for this gene.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number EC 3.1.1.4; EF; Enhancing factor; eted enzyme type IIA phospholipase A2; GIIC sPLA2; group IIA phospholipase A2; modifier of Min1; Mom1; non-pancreatic secreted type II phospholipase A2; non-pancreatic secretory phospholipase A2; NPS-PLA2; phosphatidylcholine 2-acylhydrolase 2 A; phospholipase A2 group IIA; phospholipase A2, group IIA (platelets, synovial fluid); phospholipase A2, membrane associated; PLA2; PLA2B; PLA2BPLA2; PLA2G2A; PLA2L; PLA2LPLA2S; PLA2S; PLAS1; platelet phospholipase A2; RASF-A; secretory group II phospholipase A2; sPLA2; sPla2-IIA
Assay Range 32 to 8,000 pg/mL
Assay Sensitivity 32 pg/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 5320
Gene Symbol PLA2G2A
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Target PLA2G2A
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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