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  5. 16801586

Proteintech™ Human PIGF ELISA Kit

Product Code. 16801586 Shop All Proteintech Products
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Target Species:
Human
Unit Size:
96 tests
Product Code. Target Species unitSize
16801586 Human 96 tests
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Product Code. 16801586

Brand: Proteintech™ KE0017396T

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This item is not returnable. View return policy

Description

Solid phase sandwich ELISA used to detect and quantify protein levels of endogenous PIGF

  • Recognizes human PIGF
  • PIGF (Placenta growth factor, PGF) is an angiogenic factor belonging to vascular endothelial growth factor family
    • Located on chromosome 14q14, encodes four isoforms of PIGF
    • Protein secreted as glycosylated homodimer
    • PIGF-1 and 3 are diffusible isoforms, PIGF-2 and 4 have heparin binding domains
    • PIGF identified in placenta, regulates growth and differentiation of trophoblasts, expressed in umbilical vein endothelial cells and non-placental tissues (thyroid gland and developing lung tissue)
    • Has angiogenic properties, enhancing survival, growth and migration of endothelial cells in vitro and promotes vessel formation in certain in vivo models
    • Potential biomarker for preeclampsia

Process:

  • Antibody specific for PIGF pre-coated onto microwells
  • PIGF protein in samples captured by the coated antibody after incubation
  • After extensive washing, another antibody of biotinylated specific for PIGF is added to detect captured PIGF protein
  • For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent
  • Solution containing sulfuric acid used to stop color development and color intensity
    • Proportional to quantity of bound protein, measurable at 450 nm with correction wavelength set at 630 nm

Specifications

Accession Number BC029408
Assay Range 125 to 8000 pg/mL
Assay Sensitivity 16.8 pg/mL
Certifications/Compliance ISO 13485
Detection Method Sandwich ELISA, Double Antibody
Immunoassay Kit Format Coated ELISA Kit
Sample Type Plasma, Serum
Substrate DAB
Target Species Human
Gene ID (Entrez) 5281
Host Species Human
Immunoassay Kit Grade Solid
Interassay CV <10%
Intraassay CV <10%
Shipping Condition Blue ice
Storage Requirements -20°C to +4°C
Total Assay Time 4.5 hr.
UniProt ID P49763
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Safety and Handling

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Product Identifier
  • 96TEST Rat Fabp1 ELISA Kit
 Signal Word
  • Warning
 Hazard Category
  • Serious eye damage/eye irritation Category 1
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity Category 3
 Hazard Statement
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H318-Causes serious eye damage.
  • H319-Causes serious eye irritation.
  • H332-Harmful if inhaled.
  • H335-May cause respiratory irritation.
 Precautionary Statement
  • P201-Obtain special instructions before use.
  • P202-Do not handle until all safety precautions have been read and understood.
  • P234-Keep only in original container.
  • P264-Wash skin thoroughly after handling.
  • P270-Do not eat, drink or smoke when using this product.
  • P273-Avoid release to the environment.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P308+P313-IF exposed or concerned: Get medical advice/attention.
  • P330-Rinse mouth.
  • P362-Take off contaminated clothing.
  • P405-Store locked up.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : Sulphuric Acid, Tetramethylbenzidine
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