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Invitrogen™ Human Myeloperoxidase Instant ELISA™ Kit

Product Code. 15501547
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Quantity:
128 Tests
Unit Size:
128 tests
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Product Code. 15501547

Brand: Invitrogen™ BMS2038INST

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This item is not returnable. View return policy

Description

ELISA

The Human Myeloperoxidase (Hu MPO) ELISA quantitates Hu MPO in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu MPO. Principle of the method The Human MPO solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Myeloperoxidase (MPO) is a heme protein that is synthesized during myeloid differentiation and is a major component of neutrophil azurophilic granules. It is produced as a single chain precursor and subsequently cleaved into a light and heavy chain. The mature MPO is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme is responsible for producing hypohalous acids that are central to the microbicidal activity of neutrophils. Diseases that are associated with MPO include Myeloperoxidase Deficiency and Alzheimer's Disease, Familial, 1.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P05164
Assay Range 156 to 10,000 pg/mL
Assay Sensitivity 26 pg/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
Target Species Human
For Use With (Application) ELISA
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 4353
Gene Symbol MPO
Interassay CV 9.5%
Intraassay CV 6.2
Kit Contents Pre-coated 96 well Microplate, Biotinylated Detection Antibody, SAV-HRP, Sample Diluent, lyophilized, Standard (8-well strips x 4), Wash Buffer Concentrate, Substrate Solution, Sample Diluent, Stop Solution, Lysis Buffer 10x, Adhesive Plate Covers
Label or Dye HRP
Quantity 128 Tests
Regulatory Status RUO
Sample Volume Plasma 10 μL, Serum 10 μL, Supernatant 10 μL
Storage Requirements -20°C
Synonym Myeloperoxidase
Target Myeloperoxidase
Test Time 15 mins
Total Assay Time 3 hrs 10 mins
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