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  5. Invitrogen™ Human IL-12 p70 Uncoated ELISA Kit with Plates

Invitrogen™ Human IL-12 p70 Uncoated ELISA Kit with Plates

Product Code. 15521067
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Quantity:
10 x 96 Tests
2 x 96 Tests
20 x 96 Tests
Unit Size:
Pack of 10
Pack of 2
Pack of 20
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Product Code. 15521067

Brand: Invitrogen™ 88712686

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Description

ELISA

The Human Interleukin 12 p70 (IL-12 p70) Uncoated ELISA Kit contains pre-matched antibody pairs,plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-12 p70. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

IL-12 p70, also known as interleukin-12, is a cytokine that plays a critical role in the immune system's response to infections and cancer. It is produced by immune cells, including dendritic cells, macrophages, and B cells, in response to certain pathogens and tumor cells. IL-12 p70 stimulates the production of interferon-gamma and other cytokines, which promote the activation and proliferation of T cells and natural killer cells. IL-12 p70 has been studied as a potential therapeutic target for various diseases and conditions, including infectious diseases, autoimmune disorders, and cancer. It has been shown to enhance immune responses against certain infections and tumors, and to modulate inflammatory responses in autoimmune diseases.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P29459, P29460
Assay Range 4-500 pg/mL
Assay Sensitivity 4 pg/mL
Content And Storage Capture Antibody: Pre-titrated, purified antibody
Detection Antibody: Pre-titrated, biotin-conjugated antibody
Standard: Recombinant cytokine for generating standard curve and calibrating samples
ELISA/ELISPOT Coating Buffer Powder: This uncoated ELISA kit with plates may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 μM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).
Assay Diluent: 5X concentrated
Detection enzyme: Pre-titrated Avidin-HRP
Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution
Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22, -44, -76, -86)
Conjugate HRP
Immunoassay Kit Format Uncoated ELISA Kit with Plates
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
Target Species Human
For Use With (Application) ELISA
For Use With (Equipment) Absorbance Microplate Reader
Gene Alias 20C2; CLMF; CLMF p35; CLMF p40; CLMF2; cytokine; cytotoxic lymphocyte maturation factor 1, p35; cytotoxic lymphocyte maturation factor 35 kDa subunit; Cytotoxic lymphocyte maturation factor 40 kDa subunit; il 12; IL 12p70; Il12; IL-12; Il-12 p35; IL-12 p75; IL-12 subunit p35; IL-12 subunit p40; IL-12, subunit p35; IL12, subunit p40; Il-12; cytokine; IL12A; IL-12A; IL12A/B; IL12AB; IL12B; Il-12b; IL12P35; IL-12p35; Il12p40; Il-12p40; IL35 subunit; ILN; IMD28; IMD29; Interleukin; interleukin 12 35 kDa subunit; interleukin 12 35kDa subunit; interleukin 12 40 kDa subunit; interleukin 12 40kDa subunit; interleukin 12 p35 subunit; interleukin 12 p40 subunit precursor; interleukin 12 polypeptide 35; interleukin 12, p35; interleukin 12, p40; interleukin 12A; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); interleukin 12B; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); interleukin 12p35 subunit precursor; interleukin-12 alpha chain; interleukin-12 alpha p40 subunit; interleukin-12 beta chain; interleukin-12 beta p35 subunit; interleukin-12 p35 subunit; interleukin-12 p35 subunit precursor; interleukin-12 p40; interleukin-12 p40 subunit; interleukin-12 p40 subunit precursor; Interleukin-12 p70; interleukin-12 subunit alpha; Interleukin-12 subunit beta; interleukin-23 p40 subunit; Ll12a; MGC151228; MGC151232; natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35; natural killer cell stimulatory factor, 40 kD subunit; NF cell stimulatory factor chain 1; NFSK; NK cell stimulatory factor chain 1; NK cell stimulatory factor chain 2; NKSF; NKSF1; NKSF2; P35; p4020C2; CLMF; CLMF p35; CLMF p40; CLMF2; cytokine; cytotoxic lymphocyte maturation factor 1, p35; cytotoxic lymphocyte maturation factor 35 kDa subunit; Cytotoxic lymphocyte maturation factor 40 kDa subunit; il 12; IL 12p70; Il12; IL-12; Il-12 p35; IL-12 p75; IL-12 subunit p35; IL-12 subunit p40; IL-12, subunit p35; IL12, subunit p40; Il-12; cytokine; IL12A; IL-12A; IL12A/B; IL12AB; IL12B; Il-12b; IL12P35; IL-12p35; Il12p40; Il-12p40; IL35 subunit; ILN; IMD28; IMD29; Interleukin; interleukin 12 35 kDa subunit; interleukin 12 35kDa subunit; interleukin 12 40 kDa subunit; interleukin 12 40kDa subunit; interleukin 12 p35 subunit; interleukin 12 p40 subunit precursor; interleukin 12 polypeptide 35; interleukin 12, p35; interleukin 12, p40; interleukin 12A; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); interleukin 12B; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); interleukin 12p35 subunit precursor; interleukin-12 alpha chain; interleukin-12 alpha p40 subunit; interleukin-12 beta chain; interleukin-12 beta p35 subunit; interleukin-12 p35 subunit; interleukin-12 p35 subunit precursor; interleukin-12 p40; interleukin-12 p40 subunit; interleukin-12 p40 subunit precursor; Interleukin-12 p70; interleukin-12 subunit alpha; Interleukin-12 subunit beta; interleukin-23 p40 subunit; Ll12a; MGC151228; MGC151232; natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35; natural killer cell stimulatory factor, 40 kD subunit; NF cell stimulatory factor chain 1; NFSK; NK cell stimulatory factor chain 1; NK cell stimulatory factor chain 2; NKSF; NKSF1; NKSF2; P35; p40; AA690181; IL-15RA;AI503618; IL-15
Gene ID (Entrez) 3592, 3593
Gene Symbol IL12A, Il12b
Kit Contents Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, HRP-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86), Purified antibody, Detection Antibody: Pre-titrated, HRP-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
Label or Dye Biotin
Quantity 10 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Storage Requirements 2-8°C
Synonym Interleukin-12, IL12, IL23
Target IL-12 p70
Test Time 1 hr 45 min
Total Assay Time 24 hr 30 min
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