Invitrogen™ Human Cu/ZnSOD ELISA Kit

Includes

Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human Cu/ZnSOD
HRP-Conjugate anti-human Cu/ZnSOD monoclonal antibody
Human Cu/ZnSOD Standard, 5ng/mL
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Phosphate Buffered Saline Concentrate (PBS) 20x
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Adhesive Films

ELISA

The Human Cu/Zn Superoxide Dismutase(Hu Cu/ZnSOD)) ELISA quantitates Hu Cu/ZnSOD in human serum, plasma, amniotic fluid, urine, fetal umbilical vein blood, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Cu/ZnSOD. Principle of the method The Human Cu/ZnSOD solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.