Invitrogen™ MCP-3/CCL7 Human Uncoated ELISA Kit with Plates

Includes

• Capture Antibody Concentrate
• Detection Antibody Concentrate
• Human CCL7( MCP-3) Standard (lyophilized): 100pg/mL upon reconstitution
• Coating Buffer Concentrate
• Assay Buffer A Concentrate (PBS with 1% Tween 20 and 10% BSA)
• Sample Diluent Z
• pre-titrated Streptavidin-HRP
• Tetramethylbenzidine (TMB) Substrate Solution

Uncoated ELISA Kit with Plates

The Human Monocyte Chemoattractant Protein 3 (CCL7) MCP-3) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human MCP-3. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.